The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on Listeria monocytogenes’ Survival

In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on L. monocytogenes were evaluated. The results showed that the strains of L. monocytogenes were killed completely within 30 s by SAEW whose available chlor...

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Autores principales:	Huiying Li, Duo Liang, Jin Huang, Chaojing Cui, Huan Rao, Dandan Zhao, Jianxiong Hao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	slightly acidic electrolyzed water <i>Listeria monocytogenes</i> bactericidal mechanism food safety Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/bf8adc04be954e47ae50d7c147589381
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spelling	oai:doaj.org-article:bf8adc04be954e47ae50d7c1475893812021-11-25T17:34:01ZThe Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival10.3390/foods101126712304-8158https://doaj.org/article/bf8adc04be954e47ae50d7c1475893812021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2671https://doaj.org/toc/2304-8158In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on <i>L. monocytogenes</i> were evaluated. The results showed that the strains of <i>L. monocytogenes</i> were killed completely within 30 s by SAEW whose available chlorine concentration (ACC) was higher than 12 mg/L, and it was confirmed that ACC is the main factor affecting the disinfection efficacy of SAEW. Moreover, our results demonstrated that SAEW could destroy the cell membrane of <i>L. monocytogenes</i>, which was observed by SEM and FT-IR, thus resulting in the leakage of intracellular substances including electrolyte, protein and nucleic acid, and DNA damage. On the other hand, the results found that SAEW could disrupt the intracellular ROS balance of <i>L. monocytogenes</i> by inhibiting the antioxidant enzyme activity, thus promoting the death of <i>L. monocytogenes.</i> In conclusion, the bactericidal mechanism of SAEW on <i>L. monocytogenes</i> was explained from two aspects including the damage of the cell membrane and the breaking of ROS balance.Huiying LiDuo LiangJin HuangChaojing CuiHuan RaoDandan ZhaoJianxiong HaoMDPI AGarticleslightly acidic electrolyzed water<i>Listeria monocytogenes</i>bactericidal mechanismfood safetyChemical technologyTP1-1185ENFoods, Vol 10, Iss 2671, p 2671 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	slightly acidic electrolyzed water <i>Listeria monocytogenes</i> bactericidal mechanism food safety Chemical technology TP1-1185
spellingShingle	slightly acidic electrolyzed water <i>Listeria monocytogenes</i> bactericidal mechanism food safety Chemical technology TP1-1185 Huiying Li Duo Liang Jin Huang Chaojing Cui Huan Rao Dandan Zhao Jianxiong Hao The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
description	In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on <i>L. monocytogenes</i> were evaluated. The results showed that the strains of <i>L. monocytogenes</i> were killed completely within 30 s by SAEW whose available chlorine concentration (ACC) was higher than 12 mg/L, and it was confirmed that ACC is the main factor affecting the disinfection efficacy of SAEW. Moreover, our results demonstrated that SAEW could destroy the cell membrane of <i>L. monocytogenes</i>, which was observed by SEM and FT-IR, thus resulting in the leakage of intracellular substances including electrolyte, protein and nucleic acid, and DNA damage. On the other hand, the results found that SAEW could disrupt the intracellular ROS balance of <i>L. monocytogenes</i> by inhibiting the antioxidant enzyme activity, thus promoting the death of <i>L. monocytogenes.</i> In conclusion, the bactericidal mechanism of SAEW on <i>L. monocytogenes</i> was explained from two aspects including the damage of the cell membrane and the breaking of ROS balance.
format	article
author	Huiying Li Duo Liang Jin Huang Chaojing Cui Huan Rao Dandan Zhao Jianxiong Hao
author_facet	Huiying Li Duo Liang Jin Huang Chaojing Cui Huan Rao Dandan Zhao Jianxiong Hao
author_sort	Huiying Li
title	The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
title_short	The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
title_full	The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
title_fullStr	The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
title_full_unstemmed	The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival
title_sort	bactericidal efficacy and the mechanism of action of slightly acidic electrolyzed water on <i>listeria monocytogenes’</i> survival
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bf8adc04be954e47ae50d7c147589381
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The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on <i>Listeria monocytogenes’</i> Survival

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