Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR

Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that...

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Autores principales:	Tomkowiak Agnieszka, Skowrońska Roksana, Kwiatek Michał, Spychała Julia, Weigt Dorota, Kurasiak-Popowska Danuta, Niemann Janetta, Mikołajczyk Sylwia, Nawracała Jerzy, Kowalczewski Przemysław Łukasz, Khan Kinza
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	common wheat leaf rust multiplex pcr puccinia recondita f. sp. tritici resistance genes ssr molecular markers Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c05f892de5a24a77b990ad9dd493a3ff
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spelling	oai:doaj.org-article:c05f892de5a24a77b990ad9dd493a3ff2021-12-05T14:10:40ZIdentification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR2391-541210.1515/biol-2021-0018https://doaj.org/article/c05f892de5a24a77b990ad9dd493a3ff2021-02-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0018https://doaj.org/toc/2391-5412Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes Lr34 and Lr46. This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker csLV was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene Lr34, gene Lr46 was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker wmc44 of gene Lr46 was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.Tomkowiak AgnieszkaSkowrońska RoksanaKwiatek MichałSpychała JuliaWeigt DorotaKurasiak-Popowska DanutaNiemann JanettaMikołajczyk SylwiaNawracała JerzyKowalczewski Przemysław ŁukaszKhan KinzaDe Gruyterarticlecommon wheatleaf rustmultiplex pcrpuccinia recondita f. sp. tritici resistance genesssr molecular markersBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 172-183 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	common wheat leaf rust multiplex pcr puccinia recondita f. sp. tritici resistance genes ssr molecular markers Biology (General) QH301-705.5
spellingShingle	common wheat leaf rust multiplex pcr puccinia recondita f. sp. tritici resistance genes ssr molecular markers Biology (General) QH301-705.5 Tomkowiak Agnieszka Skowrońska Roksana Kwiatek Michał Spychała Julia Weigt Dorota Kurasiak-Popowska Danuta Niemann Janetta Mikołajczyk Sylwia Nawracała Jerzy Kowalczewski Przemysław Łukasz Khan Kinza Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
description	Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes Lr34 and Lr46. This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker csLV was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene Lr34, gene Lr46 was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker wmc44 of gene Lr46 was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.
format	article
author	Tomkowiak Agnieszka Skowrońska Roksana Kwiatek Michał Spychała Julia Weigt Dorota Kurasiak-Popowska Danuta Niemann Janetta Mikołajczyk Sylwia Nawracała Jerzy Kowalczewski Przemysław Łukasz Khan Kinza
author_facet	Tomkowiak Agnieszka Skowrońska Roksana Kwiatek Michał Spychała Julia Weigt Dorota Kurasiak-Popowska Danuta Niemann Janetta Mikołajczyk Sylwia Nawracała Jerzy Kowalczewski Przemysław Łukasz Khan Kinza
author_sort	Tomkowiak Agnieszka
title	Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
title_short	Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
title_full	Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
title_fullStr	Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
title_full_unstemmed	Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR
title_sort	identification of leaf rust resistance genes lr34 and lr46 in common wheat (triticum aestivum l. ssp. aestivum) lines of different origin using multiplex pcr
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/c05f892de5a24a77b990ad9dd493a3ff
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Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR

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