Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies

Abstract Pre- and post-transplantation anti-MICA antibody detection development are associated with an increased rejection risk and low graft survival. We previously generated HLA class I null HEK-293T using CRISPR/Cas9, while MICA and MICB genes were removed in this study. A panel of 11 cell lines...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ji-Ho Jeon, In-Cheol Baek, Cheol-Hwa Hong, Ki Hyun Park, Hyeyoung Lee, Eun-Jee Oh, Tai-Gyu Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c13fc26bf6434a42ab6e2b7ee7419048
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c13fc26bf6434a42ab6e2b7ee7419048
record_format	dspace
spelling	oai:doaj.org-article:c13fc26bf6434a42ab6e2b7ee74190482021-12-02T18:49:29ZEstablishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies10.1038/s41598-021-95058-82045-2322https://doaj.org/article/c13fc26bf6434a42ab6e2b7ee74190482021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95058-8https://doaj.org/toc/2045-2322Abstract Pre- and post-transplantation anti-MICA antibody detection development are associated with an increased rejection risk and low graft survival. We previously generated HLA class I null HEK-293T using CRISPR/Cas9, while MICA and MICB genes were removed in this study. A panel of 11 cell lines expressing single MICA alleles was established. Anti-MICA antibody in the sera of kidney transplant patients was determined using flow cytometric method (FCM) and the Luminex method. In the 44 positive sera, the maximum FCM value was 2879 MFI compared to 28,135 MFI of Luminex method. Eleven sera (25%) were determined as positive by FCM and 32 sera (72%) were positive by the Luminex method. The sum of total MICA antigens, MICA002, 004, 009, 019, and *027 correlation showed a statistically significant between the two methods (P = 0.0412, P = 0.0476, P = 0.0019, P = 0.0098, P = 0.0467, and P = 0.0049). These results demonstrated that HEK-293T-based engineered cell lines expressing single MICA alleles were suitable for measuring specific antibodies against MICA antigens in the sera of transplant patients. Studies of antibodies to MICA antigens may help to understand responses in vivo and increase clinical relevance at the cellular level such as complement-dependent cytotoxicity.Ji-Ho JeonIn-Cheol BaekCheol-Hwa HongKi Hyun ParkHyeyoung LeeEun-Jee OhTai-Gyu KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji-Ho Jeon In-Cheol Baek Cheol-Hwa Hong Ki Hyun Park Hyeyoung Lee Eun-Jee Oh Tai-Gyu Kim Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
description	Abstract Pre- and post-transplantation anti-MICA antibody detection development are associated with an increased rejection risk and low graft survival. We previously generated HLA class I null HEK-293T using CRISPR/Cas9, while MICA and MICB genes were removed in this study. A panel of 11 cell lines expressing single MICA alleles was established. Anti-MICA antibody in the sera of kidney transplant patients was determined using flow cytometric method (FCM) and the Luminex method. In the 44 positive sera, the maximum FCM value was 2879 MFI compared to 28,135 MFI of Luminex method. Eleven sera (25%) were determined as positive by FCM and 32 sera (72%) were positive by the Luminex method. The sum of total MICA antigens, MICA002, 004, 009, 019, and *027 correlation showed a statistically significant between the two methods (P = 0.0412, P = 0.0476, P = 0.0019, P = 0.0098, P = 0.0467, and P = 0.0049). These results demonstrated that HEK-293T-based engineered cell lines expressing single MICA alleles were suitable for measuring specific antibodies against MICA antigens in the sera of transplant patients. Studies of antibodies to MICA antigens may help to understand responses in vivo and increase clinical relevance at the cellular level such as complement-dependent cytotoxicity.
format	article
author	Ji-Ho Jeon In-Cheol Baek Cheol-Hwa Hong Ki Hyun Park Hyeyoung Lee Eun-Jee Oh Tai-Gyu Kim
author_facet	Ji-Ho Jeon In-Cheol Baek Cheol-Hwa Hong Ki Hyun Park Hyeyoung Lee Eun-Jee Oh Tai-Gyu Kim
author_sort	Ji-Ho Jeon
title	Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
title_short	Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
title_full	Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
title_fullStr	Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
title_full_unstemmed	Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
title_sort	establishment of hla class i and mica/b null hek-293t panel expressing single mica alleles to detect anti-mica antibodies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c13fc26bf6434a42ab6e2b7ee7419048
work_keys_str_mv	AT jihojeon establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies AT incheolbaek establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies AT cheolhwahong establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies AT kihyunpark establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies AT hyeyounglee establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies AT eunjeeoh establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies AT taigyukim establishmentofhlaclassiandmicabnullhek293tpanelexpressingsinglemicaallelestodetectantimicaantibodies
_version_	1718377588646215680

Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies

Ejemplares similares