Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells

There are two technical impediments for using purified Wnt proteins in serum-free stem cell cultures: rapid loss of activity and toxicity of detergents to stem cell self-renewal. Here, the authors show that lipid-stabilized Wnt3a can establish long-term culture of human intestinal and liver organoid...

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Autores principales:	Nesrin Tüysüz, Louis van Bloois, Stieneke van den Brink, Harry Begthel, Monique M. A. Verstegen, Luis J. Cruz, Lijian Hui, Luc J. W. van der Laan, Jeroen de Jonge, Robert Vries, Eric Braakman, Enrico Mastrobattista, Jan J. Cornelissen, Hans Clevers, Derk ten Berge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c1491917e9fe4c3fa3f5944fd3318bf1
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spelling	oai:doaj.org-article:c1491917e9fe4c3fa3f5944fd3318bf12021-12-02T15:38:49ZLipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells10.1038/ncomms145782041-1723https://doaj.org/article/c1491917e9fe4c3fa3f5944fd3318bf12017-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms14578https://doaj.org/toc/2041-1723There are two technical impediments for using purified Wnt proteins in serum-free stem cell cultures: rapid loss of activity and toxicity of detergents to stem cell self-renewal. Here, the authors show that lipid-stabilized Wnt3a can establish long-term culture of human intestinal and liver organoids.Nesrin TüysüzLouis van BlooisStieneke van den BrinkHarry BegthelMonique M. A. VerstegenLuis J. CruzLijian HuiLuc J. W. van der LaanJeroen de JongeRobert VriesEric BraakmanEnrico MastrobattistaJan J. CornelissenHans CleversDerk ten BergeNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nesrin Tüysüz Louis van Bloois Stieneke van den Brink Harry Begthel Monique M. A. Verstegen Luis J. Cruz Lijian Hui Luc J. W. van der Laan Jeroen de Jonge Robert Vries Eric Braakman Enrico Mastrobattista Jan J. Cornelissen Hans Clevers Derk ten Berge Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
description	There are two technical impediments for using purified Wnt proteins in serum-free stem cell cultures: rapid loss of activity and toxicity of detergents to stem cell self-renewal. Here, the authors show that lipid-stabilized Wnt3a can establish long-term culture of human intestinal and liver organoids.
format	article
author	Nesrin Tüysüz Louis van Bloois Stieneke van den Brink Harry Begthel Monique M. A. Verstegen Luis J. Cruz Lijian Hui Luc J. W. van der Laan Jeroen de Jonge Robert Vries Eric Braakman Enrico Mastrobattista Jan J. Cornelissen Hans Clevers Derk ten Berge
author_facet	Nesrin Tüysüz Louis van Bloois Stieneke van den Brink Harry Begthel Monique M. A. Verstegen Luis J. Cruz Lijian Hui Luc J. W. van der Laan Jeroen de Jonge Robert Vries Eric Braakman Enrico Mastrobattista Jan J. Cornelissen Hans Clevers Derk ten Berge
author_sort	Nesrin Tüysüz
title	Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
title_short	Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
title_full	Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
title_fullStr	Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
title_full_unstemmed	Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
title_sort	lipid-mediated wnt protein stabilization enables serum-free culture of human organ stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c1491917e9fe4c3fa3f5944fd3318bf1
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Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells

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