Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
There are two technical impediments for using purified Wnt proteins in serum-free stem cell cultures: rapid loss of activity and toxicity of detergents to stem cell self-renewal. Here, the authors show that lipid-stabilized Wnt3a can establish long-term culture of human intestinal and liver organoid...
Guardado en:
Autores principales: | Nesrin Tüysüz, Louis van Bloois, Stieneke van den Brink, Harry Begthel, Monique M. A. Verstegen, Luis J. Cruz, Lijian Hui, Luc J. W. van der Laan, Jeroen de Jonge, Robert Vries, Eric Braakman, Enrico Mastrobattista, Jan J. Cornelissen, Hans Clevers, Derk ten Berge |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/c1491917e9fe4c3fa3f5944fd3318bf1 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Assessing the origin of high-grade serous ovarian cancer using CRISPR-modification of mouse organoids
por: Kadi Lõhmussaar, et al.
Publicado: (2020) -
The leukemia-associated Mllt10/Af10-Dot1l are Tcf4/β-catenin coactivators essential for intestinal homeostasis.
por: Tokameh Mahmoudi, et al.
Publicado: (2010) -
Anti-Sclerostin antibody inhibits internalization of Sclerostin and Sclerostin-mediated antagonism of Wnt/LRP6 signaling.
por: Maarten van Dinther, et al.
Publicado: (2013) -
Epigenetic changes in Basal Cell Carcinoma affect SHH and WNT signaling components.
por: Tjinta Brinkhuizen, et al.
Publicado: (2012) -
Precancerous liver diseases do not cause increased mutagenesis in liver stem cells
por: Luan Nguyen, et al.
Publicado: (2021)