A high-throughput assay for quantitative measurement of PCR errors

Abstract The accuracy with which DNA polymerase can replicate a template DNA sequence is an extremely important property that can vary by an order of magnitude from one enzyme to another. The rate of nucleotide misincorporation is shaped by multiple factors, including PCR conditions and proofreading...

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Autores principales: Dmitriy A. Shagin, Irina A. Shagina, Andrew R. Zaretsky, Ekaterina V. Barsova, Ilya V. Kelmanson, Sergey Lukyanov, Dmitriy M. Chudakov, Mikhail Shugay
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/c1694952e05043f282fa23db7a93a02d
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