Altered conformational sampling along an evolutionary trajectory changes the catalytic activity of an enzyme

Cyclohexadienyl dehydratase (CDT) evolved from a cationic amino acid binding protein ancestor without enzymatic activity (AncCDT-1) via a series of intermediates. Here, the authors combine EPR, X-ray crystallography and MD simulations to study the structural dynamics of these evolutionary intermedia...

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Auteurs principaux: Joe A. Kaczmarski, Mithun C. Mahawaththa, Akiva Feintuch, Ben E. Clifton, Luke A. Adams, Daniella Goldfarb, Gottfried Otting, Colin J. Jackson
Format: article
Langue:EN
Publié: Nature Portfolio 2020
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Accès en ligne:https://doaj.org/article/c19f20f6641e4d50a18da243f7703954
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