BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.

Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4Rα-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karen J Bryson, Owain R Millington, Thabang Mokgethi, H Adrienne McGachy, Frank Brombacher, James Alexander
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/c1bcd7b868c747fbbf2c5478bf3bef93
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1bcd7b868c747fbbf2c5478bf3bef93
record_format	dspace
spelling	oai:doaj.org-article:c1bcd7b868c747fbbf2c5478bf3bef932021-11-18T09:13:38ZBALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.1935-27271935-273510.1371/journal.pntd.0000930https://doaj.org/article/c1bcd7b868c747fbbf2c5478bf3bef932011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21245915/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4Rα-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional target(s) for IL-4/IL-13 inducing non-healing disease, the course of L. mexicana infection was monitored in mice lacking IL-4Rα expression in specific cellular compartments. A deficiency of IL-4Rα expression on macrophages/neutrophils (in LysM(cre)IL-4Rα(-/lox) animals) had minimal effect on the outcome of L. mexicana infection compared with control (IL-4Rα(-/flox)) mice. In contrast, CD4(+) T cell specific (Lck(cre)IL-4Rα(-/lox)) IL-4Rα(-/-) mice infected with L. mexicana developed small lesions, which subsequently healed in female mice, but persisted in adult male mice. While a strong Th1 response was manifest in both male and female CD4(+) T cell specific IL-4Rα(-/-) mice infected with L. mexicana, induction of IL-4 was manifest in males but not females, independently of CD4(+) T cell IL-4 responsiveness. Similar results were obtained using pan-T cell specific (iLck(cre)IL-4Rα(-/lox)) IL-4Rα(-/-) mice. Collectively these data demonstrate that upon infection with L. mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4.Karen J BrysonOwain R MillingtonThabang MokgethiH Adrienne McGachyFrank BrombacherJames AlexanderPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 5, Iss 1, p e930 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Karen J Bryson Owain R Millington Thabang Mokgethi H Adrienne McGachy Frank Brombacher James Alexander BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.
description	Immunologically intact BALB/c mice infected with Leishmania mexicana develop non-healing progressively growing lesions associated with a biased Th2 response while similarly infected IL-4Rα-deficient mice fail to develop lesions and develop a robust Th1 response. In order to determine the functional target(s) for IL-4/IL-13 inducing non-healing disease, the course of L. mexicana infection was monitored in mice lacking IL-4Rα expression in specific cellular compartments. A deficiency of IL-4Rα expression on macrophages/neutrophils (in LysM(cre)IL-4Rα(-/lox) animals) had minimal effect on the outcome of L. mexicana infection compared with control (IL-4Rα(-/flox)) mice. In contrast, CD4(+) T cell specific (Lck(cre)IL-4Rα(-/lox)) IL-4Rα(-/-) mice infected with L. mexicana developed small lesions, which subsequently healed in female mice, but persisted in adult male mice. While a strong Th1 response was manifest in both male and female CD4(+) T cell specific IL-4Rα(-/-) mice infected with L. mexicana, induction of IL-4 was manifest in males but not females, independently of CD4(+) T cell IL-4 responsiveness. Similar results were obtained using pan-T cell specific (iLck(cre)IL-4Rα(-/lox)) IL-4Rα(-/-) mice. Collectively these data demonstrate that upon infection with L. mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4.
format	article
author	Karen J Bryson Owain R Millington Thabang Mokgethi H Adrienne McGachy Frank Brombacher James Alexander
author_facet	Karen J Bryson Owain R Millington Thabang Mokgethi H Adrienne McGachy Frank Brombacher James Alexander
author_sort	Karen J Bryson
title	BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.
title_short	BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.
title_full	BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.
title_fullStr	BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.
title_full_unstemmed	BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.
title_sort	balb/c mice deficient in cd4 t cell il-4rα expression control leishmania mexicana load although female but not male mice develop a healer phenotype.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c1bcd7b868c747fbbf2c5478bf3bef93
work_keys_str_mv	AT karenjbryson balbcmicedeficientincd4tcellil4raexpressioncontrolleishmaniamexicanaloadalthoughfemalebutnotmalemicedevelopahealerphenotype AT owainrmillington balbcmicedeficientincd4tcellil4raexpressioncontrolleishmaniamexicanaloadalthoughfemalebutnotmalemicedevelopahealerphenotype AT thabangmokgethi balbcmicedeficientincd4tcellil4raexpressioncontrolleishmaniamexicanaloadalthoughfemalebutnotmalemicedevelopahealerphenotype AT hadriennemcgachy balbcmicedeficientincd4tcellil4raexpressioncontrolleishmaniamexicanaloadalthoughfemalebutnotmalemicedevelopahealerphenotype AT frankbrombacher balbcmicedeficientincd4tcellil4raexpressioncontrolleishmaniamexicanaloadalthoughfemalebutnotmalemicedevelopahealerphenotype AT jamesalexander balbcmicedeficientincd4tcellil4raexpressioncontrolleishmaniamexicanaloadalthoughfemalebutnotmalemicedevelopahealerphenotype
_version_	1718420988656353280

BALB/c mice deficient in CD4 T cell IL-4Rα expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype.

Ejemplares similares