Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA

Abstract β-lactam antibiotics target penicillin-binding proteins (PBPs) preventing peptidoglycan synthesis and this inhibition is circumvented in methicillin resistant Staphylococcus aureus (MRSA) strains through the expression of an additional PBP, named PBP2A. This enzyme is encoded by the mecA ge...

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Autores principales:	Nathalie T. Reichmann, Mariana G. Pinho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1c1b73a4ab645769d8870ad90f7cf3b
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spelling	oai:doaj.org-article:c1c1b73a4ab645769d8870ad90f7cf3b2021-12-02T11:53:05ZRole of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA10.1038/s41598-017-06329-22045-2322https://doaj.org/article/c1c1b73a4ab645769d8870ad90f7cf3b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06329-2https://doaj.org/toc/2045-2322Abstract β-lactam antibiotics target penicillin-binding proteins (PBPs) preventing peptidoglycan synthesis and this inhibition is circumvented in methicillin resistant Staphylococcus aureus (MRSA) strains through the expression of an additional PBP, named PBP2A. This enzyme is encoded by the mecA gene located within the Staphylococcal Chromosome Cassette mec (SCCmec) mobile genetic element, of which there are 12 types described to date. Previous investigations aimed at analysing the synergistic activity of two β-lactams, oxacillin and cefoxitin, found that SCCmec type IV community-acquired MRSA strains exhibited increased susceptibility to oxacillin in the presence of cefoxitin, while hospital-acquired MRSA strains were unaffected. However, it is not clear if these differences in β-lactam resistance are indeed a consequence of the presence of the different SCCmec types. To address this question, we have exchanged the SCCmec type I in COL (HA-MRSA) for the SCCmec type IV from MW2 (CA-MRSA). This exchange did not decrease the resistance of COL against oxacillin and cefoxitin, as observed in MW2, indicating that genetic features residing outside of the SCCmec element are likely to be responsible for the discrepancy in oxacillin and cefoxitin synergy against these MRSA strains.Nathalie T. ReichmannMariana G. PinhoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathalie T. Reichmann Mariana G. Pinho Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
description	Abstract β-lactam antibiotics target penicillin-binding proteins (PBPs) preventing peptidoglycan synthesis and this inhibition is circumvented in methicillin resistant Staphylococcus aureus (MRSA) strains through the expression of an additional PBP, named PBP2A. This enzyme is encoded by the mecA gene located within the Staphylococcal Chromosome Cassette mec (SCCmec) mobile genetic element, of which there are 12 types described to date. Previous investigations aimed at analysing the synergistic activity of two β-lactams, oxacillin and cefoxitin, found that SCCmec type IV community-acquired MRSA strains exhibited increased susceptibility to oxacillin in the presence of cefoxitin, while hospital-acquired MRSA strains were unaffected. However, it is not clear if these differences in β-lactam resistance are indeed a consequence of the presence of the different SCCmec types. To address this question, we have exchanged the SCCmec type I in COL (HA-MRSA) for the SCCmec type IV from MW2 (CA-MRSA). This exchange did not decrease the resistance of COL against oxacillin and cefoxitin, as observed in MW2, indicating that genetic features residing outside of the SCCmec element are likely to be responsible for the discrepancy in oxacillin and cefoxitin synergy against these MRSA strains.
format	article
author	Nathalie T. Reichmann Mariana G. Pinho
author_facet	Nathalie T. Reichmann Mariana G. Pinho
author_sort	Nathalie T. Reichmann
title	Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
title_short	Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
title_full	Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
title_fullStr	Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
title_full_unstemmed	Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
title_sort	role of sccmec type in resistance to the synergistic activity of oxacillin and cefoxitin in mrsa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c1c1b73a4ab645769d8870ad90f7cf3b
work_keys_str_mv	AT nathalietreichmann roleofsccmectypeinresistancetothesynergisticactivityofoxacillinandcefoxitininmrsa AT marianagpinho roleofsccmectypeinresistancetothesynergisticactivityofoxacillinandcefoxitininmrsa
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Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA

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