Digital logic circuits in yeast with CRISPR-dCas9 NOR gates

The leakiness of commonly used genetic components can make the construction of complex synthetic circuits difficult. Here the authors construct NOR gate architecture, using dCas9 fused to the chromatin remodeller Mxi1, that can be wired together into complex circuits.

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Bibliographic Details
Main Authors:	Miles W. Gander, Justin D. Vrana, William E. Voje, James M. Carothers, Eric Klavins
Format:	article
Language:	EN
Published:	Nature Portfolio 2017
Subjects:	Science Q
Online Access:	https://doaj.org/article/c217f5f654de4540a39993619de94545
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spelling	oai:doaj.org-article:c217f5f654de4540a39993619de945452021-12-02T14:40:37ZDigital logic circuits in yeast with CRISPR-dCas9 NOR gates10.1038/ncomms154592041-1723https://doaj.org/article/c217f5f654de4540a39993619de945452017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15459https://doaj.org/toc/2041-1723The leakiness of commonly used genetic components can make the construction of complex synthetic circuits difficult. Here the authors construct NOR gate architecture, using dCas9 fused to the chromatin remodeller Mxi1, that can be wired together into complex circuits.Miles W. GanderJustin D. VranaWilliam E. VojeJames M. CarothersEric KlavinsNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Miles W. Gander Justin D. Vrana William E. Voje James M. Carothers Eric Klavins Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
description	The leakiness of commonly used genetic components can make the construction of complex synthetic circuits difficult. Here the authors construct NOR gate architecture, using dCas9 fused to the chromatin remodeller Mxi1, that can be wired together into complex circuits.
format	article
author	Miles W. Gander Justin D. Vrana William E. Voje James M. Carothers Eric Klavins
author_facet	Miles W. Gander Justin D. Vrana William E. Voje James M. Carothers Eric Klavins
author_sort	Miles W. Gander
title	Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
title_short	Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
title_full	Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
title_fullStr	Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
title_full_unstemmed	Digital logic circuits in yeast with CRISPR-dCas9 NOR gates
title_sort	digital logic circuits in yeast with crispr-dcas9 nor gates
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c217f5f654de4540a39993619de94545
work_keys_str_mv	AT mileswgander digitallogiccircuitsinyeastwithcrisprdcas9norgates AT justindvrana digitallogiccircuitsinyeastwithcrisprdcas9norgates AT williamevoje digitallogiccircuitsinyeastwithcrisprdcas9norgates AT jamesmcarothers digitallogiccircuitsinyeastwithcrisprdcas9norgates AT ericklavins digitallogiccircuitsinyeastwithcrisprdcas9norgates
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Digital logic circuits in yeast with CRISPR-dCas9 NOR gates

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