Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the ge...
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oai:doaj.org-article:c226ec2616a248d788bc15a97b4f16262021-11-12T04:47:40ZGenetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers2666-166710.1016/j.xpro.2021.100939https://doaj.org/article/c226ec2616a248d788bc15a97b4f16262021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006456https://doaj.org/toc/2666-1667Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis.For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).Nicole AmbergSimon HippenmeyerElsevierarticleSingle CellDevelopmental biologyGeneticsMicroscopyModel OrganismsMolecular BiologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100939- (2021) |
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Single Cell Developmental biology Genetics Microscopy Model Organisms Molecular Biology Science (General) Q1-390 |
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Single Cell Developmental biology Genetics Microscopy Model Organisms Molecular Biology Science (General) Q1-390 Nicole Amberg Simon Hippenmeyer Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
description |
Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis.For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021). |
format |
article |
author |
Nicole Amberg Simon Hippenmeyer |
author_facet |
Nicole Amberg Simon Hippenmeyer |
author_sort |
Nicole Amberg |
title |
Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
title_short |
Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
title_full |
Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
title_fullStr |
Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
title_full_unstemmed |
Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
title_sort |
genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doaj.org/article/c226ec2616a248d788bc15a97b4f1626 |
work_keys_str_mv |
AT nicoleamberg geneticmosaicdissectionofcandidategenesinmiceusingmosaicanalysiswithdoublemarkers AT simonhippenmeyer geneticmosaicdissectionofcandidategenesinmiceusingmosaicanalysiswithdoublemarkers |
_version_ |
1718431256668012544 |