Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers

Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the ge...

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Autores principales:	Nicole Amberg, Simon Hippenmeyer
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Single Cell Developmental biology Genetics Microscopy Model Organisms Molecular Biology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/c226ec2616a248d788bc15a97b4f1626
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spelling	oai:doaj.org-article:c226ec2616a248d788bc15a97b4f16262021-11-12T04:47:40ZGenetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers2666-166710.1016/j.xpro.2021.100939https://doaj.org/article/c226ec2616a248d788bc15a97b4f16262021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006456https://doaj.org/toc/2666-1667Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis.For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).Nicole AmbergSimon HippenmeyerElsevierarticleSingle CellDevelopmental biologyGeneticsMicroscopyModel OrganismsMolecular BiologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100939- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Single Cell Developmental biology Genetics Microscopy Model Organisms Molecular Biology Science (General) Q1-390
spellingShingle	Single Cell Developmental biology Genetics Microscopy Model Organisms Molecular Biology Science (General) Q1-390 Nicole Amberg Simon Hippenmeyer Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
description	Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis.For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).
format	article
author	Nicole Amberg Simon Hippenmeyer
author_facet	Nicole Amberg Simon Hippenmeyer
author_sort	Nicole Amberg
title	Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_short	Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_full	Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_fullStr	Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_full_unstemmed	Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_sort	genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c226ec2616a248d788bc15a97b4f1626
work_keys_str_mv	AT nicoleamberg geneticmosaicdissectionofcandidategenesinmiceusingmosaicanalysiswithdoublemarkers AT simonhippenmeyer geneticmosaicdissectionofcandidategenesinmiceusingmosaicanalysiswithdoublemarkers
_version_	1718431256668012544

Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers

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