Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification - increased discrimination of closely related species
Abstract The aim of this study was to develop an easy-to-use culture-free diagnostic method based on next generation sequencing (NGS) of PCR amplification products encompassing whole 16S-23S rRNA region to improve the resolution of bacterial species identification. To determine the resolution of the...
Saved in:
Main Authors: | Artur J. Sabat, Evert van Zanten, Viktoria Akkerboom, Guido Wisselink, Kees van Slochteren, Richard F. de Boer, Ron Hendrix, Alexander W. Friedrich, John W. A. Rossen, Anna M. D. (Mirjam) Kooistra-Smid |
---|---|
Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2017
|
Subjects: | |
Online Access: | https://doaj.org/article/c3917d4f1aa84d428f1e7e27ec879c46 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Organism-specific rRNA capture system for application in next-generation sequencing.
by: Sai-Kam Li, et al.
Published: (2013) -
Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections.
by: Stephen J Salipante, et al.
Published: (2013) -
The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing
by: Ping Sun, et al.
Published: (2021) -
Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene
by: Almando Geraldi, et al.
Published: (2021) -
Detection of 16S rRNA and KPC Genes from Complex Matrix Utilizing a Molecular Inversion Probe Assay for Next-Generation Sequencing
by: Christopher P. Stefan, et al.
Published: (2018)