Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey

Introduction. Whey is a by-product and an excellent source of proteins that is rather aggressive due to a large amount of organic substances it contains. The electro-activation of whey applied in the experiments is a wasteless method that allows the va-lorification of all whey components. β-lactoglo...

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Autores principales:	Mircea BOLOGA, Elvira VRABIE, Irina PALADII, Olga ILIASENCO, Tatiana STEPURINA, Valeria VRABIE, Albert POLICARPOV, Catalina SPRINCEAN
Formato:	article
Lenguaje:	EN FR RO RU
Publicado:	Asociatia de Biosiguranta si Biosecuritate 2021
Materias:	protein-mineral concentrates β-lactoglobulin electroactivation Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c47d449043bd430c9d3845ddc950e14f
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spelling	oai:doaj.org-article:c47d449043bd430c9d3845ddc950e14f2021-12-02T14:22:43ZPeculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey10.38045/ohrm.2021.1.062887-34582587-3466https://doaj.org/article/c47d449043bd430c9d3845ddc950e14f2021-01-01T00:00:00Zhttps://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/94/71https://doaj.org/toc/2887-3458https://doaj.org/toc/2587-3466Introduction. Whey is a by-product and an excellent source of proteins that is rather aggressive due to a large amount of organic substances it contains. The electro-activation of whey applied in the experiments is a wasteless method that allows the va-lorification of all whey components. β-lactoglobulin (β-Lg) makes up 50% of the whey proteins and 12% of the total protein content in milk. Material and methods. The recovery of β-Lg in protein-mineral concentrates (PMC) by electro-activation processing of different types of whey with different initial protein content was investigated in seven configurations. The recovery of protein fractions in the PMCs were analyzed via electrophoresis with sodium dodecyl sulfate (SDS-PAGE) and 15% non-denaturing polyacrylamide gel (PAAG). Results. Whey electro-fractionation and the obtaining of PMCs with predetermined protein content, namely of β-Lg, were studied on three whey types, processed at different treatment regimens and in seven configurations. The proper management of electroactivation by varying the treatment regimens will allow the electro-fractionation of different types of dairy by-products. Conclusions. The maximum amount of β-Lg recovered in PMCs on electroactivation is 66-71% depending on the processed whey and on the treatment regimens. Obviously, the extraction of β-Lg from initially lower protein content shows a higher recovery degree of β-Lg. The registered temperatures allows formation of PMCs without thermal denaturation.Mircea BOLOGAElvira VRABIEIrina PALADIIOlga ILIASENCOTatiana STEPURINAValeria VRABIEAlbert POLICARPOVCatalina SPRINCEANAsociatia de Biosiguranta si Biosecuritatearticleprotein-mineral concentratesβ-lactoglobulinelectroactivationMedicineRScienceQENFRRORUOne Health & Risk Management, Vol 2, Iss 1, Pp 52-68 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR RO RU
topic	protein-mineral concentrates β-lactoglobulin electroactivation Medicine R Science Q
spellingShingle	protein-mineral concentrates β-lactoglobulin electroactivation Medicine R Science Q Mircea BOLOGA Elvira VRABIE Irina PALADII Olga ILIASENCO Tatiana STEPURINA Valeria VRABIE Albert POLICARPOV Catalina SPRINCEAN Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
description	Introduction. Whey is a by-product and an excellent source of proteins that is rather aggressive due to a large amount of organic substances it contains. The electro-activation of whey applied in the experiments is a wasteless method that allows the va-lorification of all whey components. β-lactoglobulin (β-Lg) makes up 50% of the whey proteins and 12% of the total protein content in milk. Material and methods. The recovery of β-Lg in protein-mineral concentrates (PMC) by electro-activation processing of different types of whey with different initial protein content was investigated in seven configurations. The recovery of protein fractions in the PMCs were analyzed via electrophoresis with sodium dodecyl sulfate (SDS-PAGE) and 15% non-denaturing polyacrylamide gel (PAAG). Results. Whey electro-fractionation and the obtaining of PMCs with predetermined protein content, namely of β-Lg, were studied on three whey types, processed at different treatment regimens and in seven configurations. The proper management of electroactivation by varying the treatment regimens will allow the electro-fractionation of different types of dairy by-products. Conclusions. The maximum amount of β-Lg recovered in PMCs on electroactivation is 66-71% depending on the processed whey and on the treatment regimens. Obviously, the extraction of β-Lg from initially lower protein content shows a higher recovery degree of β-Lg. The registered temperatures allows formation of PMCs without thermal denaturation.
format	article
author	Mircea BOLOGA Elvira VRABIE Irina PALADII Olga ILIASENCO Tatiana STEPURINA Valeria VRABIE Albert POLICARPOV Catalina SPRINCEAN
author_facet	Mircea BOLOGA Elvira VRABIE Irina PALADII Olga ILIASENCO Tatiana STEPURINA Valeria VRABIE Albert POLICARPOV Catalina SPRINCEAN
author_sort	Mircea BOLOGA
title	Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
title_short	Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
title_full	Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
title_fullStr	Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
title_full_unstemmed	Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
title_sort	peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey
publisher	Asociatia de Biosiguranta si Biosecuritate
publishDate	2021
url	https://doaj.org/article/c47d449043bd430c9d3845ddc950e14f
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Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey

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