Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats

Abstract Doxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. This study investigated the benefi...

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Autores principales:	Godwin Adakole Ujah, Victor Udo Nna, Joseph Bagi Suleiman, Chinedum Eleazu, Chukwuemeka Nwokocha, Joy Assima Rebene, Michael Umana Imowo, Emmanuel Ochui Obi, Charlette Amachree, Evarest Chigozie Udechukwu, Mahaneem Mohamed
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c48c7161aa3b4abfbc4b34755504fb86
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spelling	oai:doaj.org-article:c48c7161aa3b4abfbc4b34755504fb862021-12-02T15:53:03ZTert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats10.1038/s41598-021-85026-72045-2322https://doaj.org/article/c48c7161aa3b4abfbc4b34755504fb862021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85026-7https://doaj.org/toc/2045-2322Abstract Doxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. This study investigated the benefits of the antioxidant, tert-butylhydroquinone (tBHQ), on testicular toxicity following DOX therapy. Twenty-four adult male albino rats were assigned randomly into four groups (n = 6), namely: normal control (NC), tBHQ, DOX and tBHQ + DOX groups. tBHQ (50 mg/kg body weight in 1% DMSO) was administered orally for 14 consecutive days, while a single DOX dose (7 mg/kg body weight) was administered intraperitoneally on Day 8. DOX decreased sperm count, motility and viability, and decreased the levels of steroidogenesis-related proteins, and reproductive hormones. Furthermore, DOX decreased the expression of antioxidant cytoprotective genes, and decreased the protein level of proliferating cell nuclear antigen in the testis. Conversely, DOX increased the expression of pro-inflammatory and pro-apoptotic genes in the testis. These negative effects were ameliorated following the intervention with tBHQ. Our results suggest that tBHQ protects the testis and preserves both steroidogenesis and spermatogenesis in DOX-treated rats through the suppression of oxidative stress, inflammation and apoptosis.Godwin Adakole UjahVictor Udo NnaJoseph Bagi SuleimanChinedum EleazuChukwuemeka NwokochaJoy Assima RebeneMichael Umana ImowoEmmanuel Ochui ObiCharlette AmachreeEvarest Chigozie UdechukwuMahaneem MohamedNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Godwin Adakole Ujah Victor Udo Nna Joseph Bagi Suleiman Chinedum Eleazu Chukwuemeka Nwokocha Joy Assima Rebene Michael Umana Imowo Emmanuel Ochui Obi Charlette Amachree Evarest Chigozie Udechukwu Mahaneem Mohamed Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
description	Abstract Doxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. This study investigated the benefits of the antioxidant, tert-butylhydroquinone (tBHQ), on testicular toxicity following DOX therapy. Twenty-four adult male albino rats were assigned randomly into four groups (n = 6), namely: normal control (NC), tBHQ, DOX and tBHQ + DOX groups. tBHQ (50 mg/kg body weight in 1% DMSO) was administered orally for 14 consecutive days, while a single DOX dose (7 mg/kg body weight) was administered intraperitoneally on Day 8. DOX decreased sperm count, motility and viability, and decreased the levels of steroidogenesis-related proteins, and reproductive hormones. Furthermore, DOX decreased the expression of antioxidant cytoprotective genes, and decreased the protein level of proliferating cell nuclear antigen in the testis. Conversely, DOX increased the expression of pro-inflammatory and pro-apoptotic genes in the testis. These negative effects were ameliorated following the intervention with tBHQ. Our results suggest that tBHQ protects the testis and preserves both steroidogenesis and spermatogenesis in DOX-treated rats through the suppression of oxidative stress, inflammation and apoptosis.
format	article
author	Godwin Adakole Ujah Victor Udo Nna Joseph Bagi Suleiman Chinedum Eleazu Chukwuemeka Nwokocha Joy Assima Rebene Michael Umana Imowo Emmanuel Ochui Obi Charlette Amachree Evarest Chigozie Udechukwu Mahaneem Mohamed
author_facet	Godwin Adakole Ujah Victor Udo Nna Joseph Bagi Suleiman Chinedum Eleazu Chukwuemeka Nwokocha Joy Assima Rebene Michael Umana Imowo Emmanuel Ochui Obi Charlette Amachree Evarest Chigozie Udechukwu Mahaneem Mohamed
author_sort	Godwin Adakole Ujah
title	Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
title_short	Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
title_full	Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
title_fullStr	Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
title_full_unstemmed	Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
title_sort	tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c48c7161aa3b4abfbc4b34755504fb86
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Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats

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