Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model

Abstract Maintenance of cellular proteostasis is vital for post-mitotic cells like neurons to sustain normal physiological function and homeostasis, defects in which are established hallmarks of several age-related conditions like AD, PD, HD, and ALS. The Spatio-temporal accumulation of aggregated p...

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Autores principales:	M. J. Vijay Kumar, Devanshi Shah, Mridhula Giridharan, Niraj Yadav, Ravi Manjithaya, James P. Clement
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c60f060929594af5ae21ab4428f52e80
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spelling	oai:doaj.org-article:c60f060929594af5ae21ab4428f52e802021-12-02T15:13:06ZSpatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model10.1038/s41598-020-78850-w2045-2322https://doaj.org/article/c60f060929594af5ae21ab4428f52e802021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78850-whttps://doaj.org/toc/2045-2322Abstract Maintenance of cellular proteostasis is vital for post-mitotic cells like neurons to sustain normal physiological function and homeostasis, defects in which are established hallmarks of several age-related conditions like AD, PD, HD, and ALS. The Spatio-temporal accumulation of aggregated proteins in the form of inclusion bodies/plaques is one of the major characteristics of many neurodegenerative diseases, including Huntington’s disease (HD). Toxic accumulation of HUNTINGTIN (HTT) aggregates in neurons bring about the aberrant phenotypes of HD, including severe motor dysfunction, dementia, and cognitive impairment at the organismal level, in an age-dependent manner. In several cellular and animal models, aggrephagy induction has been shown to clear aggregate-prone proteins like HTT and ameliorate disease pathology by conferring neuroprotection. In this study, we used the mouse model of HD, R6/2, to understand the pathogenicity of mHTT aggregates, primarily focusing on autophagy dysfunction. We report that basal autophagy is not altered in R6/2 mice, whilst being functional at a steady-state level in neurons. Moreover, we tested the efficacy of a known autophagy modulator, Nilotinib (Tasigna™), presently in clinical trials for PD, and HD, in curbing mHTT aggregate growth and their potential clearance, which was ineffective in both inducing autophagy and rescuing the pathological phenotypes in R6/2 mice.M. J. Vijay KumarDevanshi ShahMridhula GiridharanNiraj YadavRavi ManjithayaJames P. ClementNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. J. Vijay Kumar Devanshi Shah Mridhula Giridharan Niraj Yadav Ravi Manjithaya James P. Clement Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
description	Abstract Maintenance of cellular proteostasis is vital for post-mitotic cells like neurons to sustain normal physiological function and homeostasis, defects in which are established hallmarks of several age-related conditions like AD, PD, HD, and ALS. The Spatio-temporal accumulation of aggregated proteins in the form of inclusion bodies/plaques is one of the major characteristics of many neurodegenerative diseases, including Huntington’s disease (HD). Toxic accumulation of HUNTINGTIN (HTT) aggregates in neurons bring about the aberrant phenotypes of HD, including severe motor dysfunction, dementia, and cognitive impairment at the organismal level, in an age-dependent manner. In several cellular and animal models, aggrephagy induction has been shown to clear aggregate-prone proteins like HTT and ameliorate disease pathology by conferring neuroprotection. In this study, we used the mouse model of HD, R6/2, to understand the pathogenicity of mHTT aggregates, primarily focusing on autophagy dysfunction. We report that basal autophagy is not altered in R6/2 mice, whilst being functional at a steady-state level in neurons. Moreover, we tested the efficacy of a known autophagy modulator, Nilotinib (Tasigna™), presently in clinical trials for PD, and HD, in curbing mHTT aggregate growth and their potential clearance, which was ineffective in both inducing autophagy and rescuing the pathological phenotypes in R6/2 mice.
format	article
author	M. J. Vijay Kumar Devanshi Shah Mridhula Giridharan Niraj Yadav Ravi Manjithaya James P. Clement
author_facet	M. J. Vijay Kumar Devanshi Shah Mridhula Giridharan Niraj Yadav Ravi Manjithaya James P. Clement
author_sort	M. J. Vijay Kumar
title	Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
title_short	Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
title_full	Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
title_fullStr	Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
title_full_unstemmed	Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model
title_sort	spatiotemporal analysis of soluble aggregates and autophagy markers in the r6/2 mouse model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c60f060929594af5ae21ab4428f52e80
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Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model

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