Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice
Abstract This study investigated the acute and subacute toxicity of the ethanolic extract (EE) and alkaloid fraction (FA) from A. nitidum. The EE was obtained from trunk bark with ethanol, FA was obtained from the fractionation of EE. To test the acute toxicity, mice were divided into four groups, a...
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2021
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oai:doaj.org-article:c663f7942fe145589bd57c0de26983f82021-12-02T15:31:36ZEvaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice10.1038/s41598-021-97637-12045-2322https://doaj.org/article/c663f7942fe145589bd57c0de26983f82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97637-1https://doaj.org/toc/2045-2322Abstract This study investigated the acute and subacute toxicity of the ethanolic extract (EE) and alkaloid fraction (FA) from A. nitidum. The EE was obtained from trunk bark with ethanol, FA was obtained from the fractionation of EE. To test the acute toxicity, mice were divided into four groups, and the negative controls received water or aqueous solution of dimethyl sulfoxide, whereas the others received EE or FA (2000 mg/kg, orally, single dose). The same controls were used in the subacute trial. However, the animals were treated for 28 days, and the dose used was 1000 mg/kg per day of EE and FA. Daily clinical evaluations of the animals were performed. At the end of the experiment, hematological, biochemical, and histopathological assessments (liver, lung, heart, and kidney) were performed. In the acute and subacute toxicity studies, mice treated with EE and FA did not show any clinical changes, there were no changes in weight gain, hematological and biochemical parameters compared to the control groups (p > 0.05). In the histopathological examination, there was no abnormality in the organs of the treated animals. Therefore, EE and FA did not produce toxic effects in mice after acute and subacute treatment.Heliton Patrick Cordovil BrígidoEverton Luiz Pompeu VarelaAntônio Rafael Quadros GomesMirian Letícia Carmo BastosAndre de Oliveira FeitosaAndrey Moacir do Rosário MarinhoLiliane Almeida CarneiroMárlia Regina Coelho-FerreiraMaria Fâni DolabelaSandro PercárioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
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Medicine R Science Q Heliton Patrick Cordovil Brígido Everton Luiz Pompeu Varela Antônio Rafael Quadros Gomes Mirian Letícia Carmo Bastos Andre de Oliveira Feitosa Andrey Moacir do Rosário Marinho Liliane Almeida Carneiro Márlia Regina Coelho-Ferreira Maria Fâni Dolabela Sandro Percário Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice |
| description |
Abstract This study investigated the acute and subacute toxicity of the ethanolic extract (EE) and alkaloid fraction (FA) from A. nitidum. The EE was obtained from trunk bark with ethanol, FA was obtained from the fractionation of EE. To test the acute toxicity, mice were divided into four groups, and the negative controls received water or aqueous solution of dimethyl sulfoxide, whereas the others received EE or FA (2000 mg/kg, orally, single dose). The same controls were used in the subacute trial. However, the animals were treated for 28 days, and the dose used was 1000 mg/kg per day of EE and FA. Daily clinical evaluations of the animals were performed. At the end of the experiment, hematological, biochemical, and histopathological assessments (liver, lung, heart, and kidney) were performed. In the acute and subacute toxicity studies, mice treated with EE and FA did not show any clinical changes, there were no changes in weight gain, hematological and biochemical parameters compared to the control groups (p > 0.05). In the histopathological examination, there was no abnormality in the organs of the treated animals. Therefore, EE and FA did not produce toxic effects in mice after acute and subacute treatment. |
| format |
article |
| author |
Heliton Patrick Cordovil Brígido Everton Luiz Pompeu Varela Antônio Rafael Quadros Gomes Mirian Letícia Carmo Bastos Andre de Oliveira Feitosa Andrey Moacir do Rosário Marinho Liliane Almeida Carneiro Márlia Regina Coelho-Ferreira Maria Fâni Dolabela Sandro Percário |
| author_facet |
Heliton Patrick Cordovil Brígido Everton Luiz Pompeu Varela Antônio Rafael Quadros Gomes Mirian Letícia Carmo Bastos Andre de Oliveira Feitosa Andrey Moacir do Rosário Marinho Liliane Almeida Carneiro Márlia Regina Coelho-Ferreira Maria Fâni Dolabela Sandro Percário |
| author_sort |
Heliton Patrick Cordovil Brígido |
| title |
Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice |
| title_short |
Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice |
| title_full |
Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice |
| title_fullStr |
Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice |
| title_full_unstemmed |
Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice |
| title_sort |
evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of aspidosperma nitidum in mice |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c663f7942fe145589bd57c0de26983f8 |
| work_keys_str_mv |
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