Performance of a scalable RNA extraction-free transcriptome profiling method for adherent cultured human cells

Abstract RNA sequencing enables high-content/high-complexity measurements in small molecule screens. Whereas the costs of DNA sequencing and RNA-seq library preparation have decreased consistently, RNA extraction remains a significant bottleneck to scalability. We evaluate the performance of a bulk...

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Auteurs principaux: Shreya Ghimire, Carley G. Stewart, Andrew L. Thurman, Alejandro A. Pezzulo
Format: article
Langue:EN
Publié: Nature Portfolio 2021
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Accès en ligne:https://doaj.org/article/c6a411eebcfd44aaae63f495fea96183
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