Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System

Abstract The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88–100...

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Autores principales:	Karim Khalil, Medhat Elayat, Elsayed Khalifa, Samer Daghash, Ahmed Elaswad, Michael Miller, Hisham Abdelrahman, Zhi Ye, Ramjie Odin, David Drescher, Khoi Vo, Kamal Gosh, William Bugg, Dalton Robinson, Rex Dunham
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7fb0daec0164530b79df13bc376fa68
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spelling	oai:doaj.org-article:c7fb0daec0164530b79df13bc376fa682021-12-02T12:30:11ZGeneration of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System10.1038/s41598-017-07223-72045-2322https://doaj.org/article/c7fb0daec0164530b79df13bc376fa682017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07223-7https://doaj.org/toc/2045-2322Abstract The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88–100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.Karim KhalilMedhat ElayatElsayed KhalifaSamer DaghashAhmed ElaswadMichael MillerHisham AbdelrahmanZhi YeRamjie OdinDavid DrescherKhoi VoKamal GoshWilliam BuggDalton RobinsonRex DunhamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karim Khalil Medhat Elayat Elsayed Khalifa Samer Daghash Ahmed Elaswad Michael Miller Hisham Abdelrahman Zhi Ye Ramjie Odin David Drescher Khoi Vo Kamal Gosh William Bugg Dalton Robinson Rex Dunham Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
description	Abstract The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88–100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.
format	article
author	Karim Khalil Medhat Elayat Elsayed Khalifa Samer Daghash Ahmed Elaswad Michael Miller Hisham Abdelrahman Zhi Ye Ramjie Odin David Drescher Khoi Vo Kamal Gosh William Bugg Dalton Robinson Rex Dunham
author_facet	Karim Khalil Medhat Elayat Elsayed Khalifa Samer Daghash Ahmed Elaswad Michael Miller Hisham Abdelrahman Zhi Ye Ramjie Odin David Drescher Khoi Vo Kamal Gosh William Bugg Dalton Robinson Rex Dunham
author_sort	Karim Khalil
title	Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
title_short	Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
title_full	Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
title_fullStr	Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
title_full_unstemmed	Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
title_sort	generation of myostatin gene-edited channel catfish (ictalurus punctatus) via zygote injection of crispr/cas9 system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c7fb0daec0164530b79df13bc376fa68
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Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System

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