An in vitro platform for engineering and harnessing modular polyketide synthases

A robust platform to study modular polyketide synthases (PKSs) in vitro is still unavailable. Here, the authors report the reconstitution of the venemycin PKS, engineer hybrid venemycin/pikromycin PKSs, and obtain much improved yields through employing the updated module boundaries.

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Autores principales:	Takeshi Miyazawa, Melissa Hirsch, Zhicheng Zhang, Adrian T. Keatinge-Clay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c81b22727cf3473480de12de0e456449
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spelling	oai:doaj.org-article:c81b22727cf3473480de12de0e4564492021-12-02T15:39:06ZAn in vitro platform for engineering and harnessing modular polyketide synthases10.1038/s41467-019-13811-02041-1723https://doaj.org/article/c81b22727cf3473480de12de0e4564492020-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13811-0https://doaj.org/toc/2041-1723A robust platform to study modular polyketide synthases (PKSs) in vitro is still unavailable. Here, the authors report the reconstitution of the venemycin PKS, engineer hybrid venemycin/pikromycin PKSs, and obtain much improved yields through employing the updated module boundaries.Takeshi MiyazawaMelissa HirschZhicheng ZhangAdrian T. Keatinge-ClayNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Takeshi Miyazawa Melissa Hirsch Zhicheng Zhang Adrian T. Keatinge-Clay An in vitro platform for engineering and harnessing modular polyketide synthases
description	A robust platform to study modular polyketide synthases (PKSs) in vitro is still unavailable. Here, the authors report the reconstitution of the venemycin PKS, engineer hybrid venemycin/pikromycin PKSs, and obtain much improved yields through employing the updated module boundaries.
format	article
author	Takeshi Miyazawa Melissa Hirsch Zhicheng Zhang Adrian T. Keatinge-Clay
author_facet	Takeshi Miyazawa Melissa Hirsch Zhicheng Zhang Adrian T. Keatinge-Clay
author_sort	Takeshi Miyazawa
title	An in vitro platform for engineering and harnessing modular polyketide synthases
title_short	An in vitro platform for engineering and harnessing modular polyketide synthases
title_full	An in vitro platform for engineering and harnessing modular polyketide synthases
title_fullStr	An in vitro platform for engineering and harnessing modular polyketide synthases
title_full_unstemmed	An in vitro platform for engineering and harnessing modular polyketide synthases
title_sort	in vitro platform for engineering and harnessing modular polyketide synthases
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c81b22727cf3473480de12de0e456449
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An in vitro platform for engineering and harnessing modular polyketide synthases

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