Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).

<h4>Background</h4>MicroRNAs (miRNAs) are short, noncoding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. The miRNAs, MIR-15a/16-1, at chromosome band 13q14 are down-regulated in the majority of patients with chronic l...

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Autores principales:	Katy Hanlon, Claudius E Rudin, Lorna W Harries
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c83ca5205432473e816dd3e33701aa01
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spelling	oai:doaj.org-article:c83ca5205432473e816dd3e33701aa012021-11-25T06:20:08ZInvestigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).1932-620310.1371/journal.pone.0007169https://doaj.org/article/c83ca5205432473e816dd3e33701aa012009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19779621/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) are short, noncoding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. The miRNAs, MIR-15a/16-1, at chromosome band 13q14 are down-regulated in the majority of patients with chronic lymphocytic leukaemia (CLL).<h4>Methodology/principal findings</h4>We have measured the expression of MIR-15a/16-1, and 92 computationally-predicted MIR-15a/16-1 target genes in CLL patients and in normal controls. We identified 35 genes that are deregulated in CLL patients, 5 of which appear to be specific targets of the MIR-15a/16-1 cluster. These targets included 2 genes (BAZ2A and RNF41) that were significantly up-regulated (p<0.05) and 3 genes (RASSF5, MKK3 and LRIG1) that were significantly down-regulated (p<0.05) in CLL patients with down-regulated MIR-15a/16-1 expression.<h4>Significance</h4>The genes identified here as being subject to MIR-15a/16-1 regulation could represent direct or indirect targets of these miRNAs. Many of these are good biological candidates for involvement in tumorigenesis and as such, may be important in the aetiology of CLL.Katy HanlonClaudius E RudinLorna W HarriesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e7169 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katy Hanlon Claudius E Rudin Lorna W Harries Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).
description	<h4>Background</h4>MicroRNAs (miRNAs) are short, noncoding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. The miRNAs, MIR-15a/16-1, at chromosome band 13q14 are down-regulated in the majority of patients with chronic lymphocytic leukaemia (CLL).<h4>Methodology/principal findings</h4>We have measured the expression of MIR-15a/16-1, and 92 computationally-predicted MIR-15a/16-1 target genes in CLL patients and in normal controls. We identified 35 genes that are deregulated in CLL patients, 5 of which appear to be specific targets of the MIR-15a/16-1 cluster. These targets included 2 genes (BAZ2A and RNF41) that were significantly up-regulated (p<0.05) and 3 genes (RASSF5, MKK3 and LRIG1) that were significantly down-regulated (p<0.05) in CLL patients with down-regulated MIR-15a/16-1 expression.<h4>Significance</h4>The genes identified here as being subject to MIR-15a/16-1 regulation could represent direct or indirect targets of these miRNAs. Many of these are good biological candidates for involvement in tumorigenesis and as such, may be important in the aetiology of CLL.
format	article
author	Katy Hanlon Claudius E Rudin Lorna W Harries
author_facet	Katy Hanlon Claudius E Rudin Lorna W Harries
author_sort	Katy Hanlon
title	Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).
title_short	Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).
title_full	Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).
title_fullStr	Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).
title_full_unstemmed	Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).
title_sort	investigating the targets of mir-15a and mir-16-1 in patients with chronic lymphocytic leukemia (cll).
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/c83ca5205432473e816dd3e33701aa01
work_keys_str_mv	AT katyhanlon investigatingthetargetsofmir15aandmir161inpatientswithchroniclymphocyticleukemiacll AT claudiuserudin investigatingthetargetsofmir15aandmir161inpatientswithchroniclymphocyticleukemiacll AT lornawharries investigatingthetargetsofmir15aandmir161inpatientswithchroniclymphocyticleukemiacll
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Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).

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