Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance

Cas13b can be harnessed to target and degrade RNA transcripts inside a cellular environment. Here the authors reprogram Cas13b to target SARSCoV-2 transcripts in infected mammalian cells and reveal its resilience to variants thanks to single mismatch tolerance.

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohamed Fareh, Wei Zhao, Wenxin Hu, Joshua M. L. Casan, Amit Kumar, Jori Symons, Jennifer M. Zerbato, Danielle Fong, Ilia Voskoboinik, Paul G. Ekert, Rajeev Rudraraju, Damian F. J. Purcell, Sharon R. Lewin, Joseph A. Trapani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c9a22d0a72d14aecb6991c4771cee7a6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c9a22d0a72d14aecb6991c4771cee7a6
record_format	dspace
spelling	oai:doaj.org-article:c9a22d0a72d14aecb6991c4771cee7a62021-12-02T16:08:13ZReprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance10.1038/s41467-021-24577-92041-1723https://doaj.org/article/c9a22d0a72d14aecb6991c4771cee7a62021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24577-9https://doaj.org/toc/2041-1723Cas13b can be harnessed to target and degrade RNA transcripts inside a cellular environment. Here the authors reprogram Cas13b to target SARSCoV-2 transcripts in infected mammalian cells and reveal its resilience to variants thanks to single mismatch tolerance.Mohamed FarehWei ZhaoWenxin HuJoshua M. L. CasanAmit KumarJori SymonsJennifer M. ZerbatoDanielle FongIlia VoskoboinikPaul G. EkertRajeev RudrarajuDamian F. J. PurcellSharon R. LewinJoseph A. TrapaniNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mohamed Fareh Wei Zhao Wenxin Hu Joshua M. L. Casan Amit Kumar Jori Symons Jennifer M. Zerbato Danielle Fong Ilia Voskoboinik Paul G. Ekert Rajeev Rudraraju Damian F. J. Purcell Sharon R. Lewin Joseph A. Trapani Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
description	Cas13b can be harnessed to target and degrade RNA transcripts inside a cellular environment. Here the authors reprogram Cas13b to target SARSCoV-2 transcripts in infected mammalian cells and reveal its resilience to variants thanks to single mismatch tolerance.
format	article
author	Mohamed Fareh Wei Zhao Wenxin Hu Joshua M. L. Casan Amit Kumar Jori Symons Jennifer M. Zerbato Danielle Fong Ilia Voskoboinik Paul G. Ekert Rajeev Rudraraju Damian F. J. Purcell Sharon R. Lewin Joseph A. Trapani
author_facet	Mohamed Fareh Wei Zhao Wenxin Hu Joshua M. L. Casan Amit Kumar Jori Symons Jennifer M. Zerbato Danielle Fong Ilia Voskoboinik Paul G. Ekert Rajeev Rudraraju Damian F. J. Purcell Sharon R. Lewin Joseph A. Trapani
author_sort	Mohamed Fareh
title	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_short	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_full	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_fullStr	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_full_unstemmed	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_sort	reprogrammed crispr-cas13b suppresses sars-cov-2 replication and circumvents its mutational escape through mismatch tolerance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c9a22d0a72d14aecb6991c4771cee7a6
work_keys_str_mv	AT mohamedfareh reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT weizhao reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT wenxinhu reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT joshuamlcasan reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT amitkumar reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT jorisymons reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT jennifermzerbato reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT daniellefong reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT iliavoskoboinik reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT paulgekert reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT rajeevrudraraju reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT damianfjpurcell reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT sharonrlewin reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT josephatrapani reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance
_version_	1718384559122284544

Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance

Ejemplares similares