Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.

The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic...

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Autores principales:	Julien Burlaud-Gaillard, Caroline Sellin, Sonia Georgeault, Rustem Uzbekov, Claude Lebos, Jean-Marc Guillaume, Philippe Roingeard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb212d60407f476fb56464201793e939
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spelling	oai:doaj.org-article:cb212d60407f476fb56464201793e9392021-11-18T08:25:43ZCorrelative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.1932-620310.1371/journal.pone.0093573https://doaj.org/article/cb212d60407f476fb56464201793e9392014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24681578/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at the cell surface through a short-lived process. For transmission electron microscopy (TEM) studies of the intracellular ultrastructure of the small number of cells releasing viral particles at a given time, we developed a new correlative microscopy method: CSEMTEM (for correlative scanning electron microscopy - transmission electron microscopy). CSEMTEM analysis suggested that chimeric flavivirus particles were released as individual particles, in small exocytosis vesicles, via a regulated secretory pathway. Our morphological findings provide new insight into interactions between flaviviruses and cells and demonstrate that CSEMTEM is a useful new method, complementary to SEM observations of biological events by intracellular TEM investigations.Julien Burlaud-GaillardCaroline SellinSonia GeorgeaultRustem UzbekovClaude LebosJean-Marc GuillaumePhilippe RoingeardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e93573 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julien Burlaud-Gaillard Caroline Sellin Sonia Georgeault Rustem Uzbekov Claude Lebos Jean-Marc Guillaume Philippe Roingeard Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
description	The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at the cell surface through a short-lived process. For transmission electron microscopy (TEM) studies of the intracellular ultrastructure of the small number of cells releasing viral particles at a given time, we developed a new correlative microscopy method: CSEMTEM (for correlative scanning electron microscopy - transmission electron microscopy). CSEMTEM analysis suggested that chimeric flavivirus particles were released as individual particles, in small exocytosis vesicles, via a regulated secretory pathway. Our morphological findings provide new insight into interactions between flaviviruses and cells and demonstrate that CSEMTEM is a useful new method, complementary to SEM observations of biological events by intracellular TEM investigations.
format	article
author	Julien Burlaud-Gaillard Caroline Sellin Sonia Georgeault Rustem Uzbekov Claude Lebos Jean-Marc Guillaume Philippe Roingeard
author_facet	Julien Burlaud-Gaillard Caroline Sellin Sonia Georgeault Rustem Uzbekov Claude Lebos Jean-Marc Guillaume Philippe Roingeard
author_sort	Julien Burlaud-Gaillard
title	Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
title_short	Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
title_full	Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
title_fullStr	Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
title_full_unstemmed	Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
title_sort	correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cb212d60407f476fb56464201793e939
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Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.

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