In vitro synthesis of gene-length single-stranded DNA

Abstract Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for...

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Auteurs principaux: Rémi Veneziano, Tyson R. Shepherd, Sakul Ratanalert, Leila Bellou, Chaoqun Tao, Mark Bathe
Format: article
Langue:EN
Publié: Nature Portfolio 2018
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Accès en ligne:https://doaj.org/article/cb86edf54d00480d9e19c68973dc90bb
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