Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.

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Detalles Bibliográficos
Autores principales: Yanhe Li, Yuteng Weng, Dandan Bai, Yanping Jia, Yingdong Liu, Yalin Zhang, Xiaochen Kou, Yanhong Zhao, Jingling Ruan, Jiayu Chen, Jiqing Yin, Hong Wang, Xiaoming Teng, Zuolin Wang, Wenqiang Liu, Shaorong Gao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/cc16067a91c84bc8a8ce6c62671c27c7
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https://doaj.org/article/cc16067a91c84bc8a8ce6c62671c27c7

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