Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.

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Autores principales:	Yanhe Li, Yuteng Weng, Dandan Bai, Yanping Jia, Yingdong Liu, Yalin Zhang, Xiaochen Kou, Yanhong Zhao, Jingling Ruan, Jiayu Chen, Jiqing Yin, Hong Wang, Xiaoming Teng, Zuolin Wang, Wenqiang Liu, Shaorong Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cc16067a91c84bc8a8ce6c62671c27c7
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spelling	oai:doaj.org-article:cc16067a91c84bc8a8ce6c62671c27c72021-12-02T18:34:03ZPrecise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation10.1038/s41467-020-18391-y2041-1723https://doaj.org/article/cc16067a91c84bc8a8ce6c62671c27c72020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18391-yhttps://doaj.org/toc/2041-1723Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.Yanhe LiYuteng WengDandan BaiYanping JiaYingdong LiuYalin ZhangXiaochen KouYanhong ZhaoJingling RuanJiayu ChenJiqing YinHong WangXiaoming TengZuolin WangWenqiang LiuShaorong GaoNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yanhe Li Yuteng Weng Dandan Bai Yanping Jia Yingdong Liu Yalin Zhang Xiaochen Kou Yanhong Zhao Jingling Ruan Jiayu Chen Jiqing Yin Hong Wang Xiaoming Teng Zuolin Wang Wenqiang Liu Shaorong Gao Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
description	Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.
format	article
author	Yanhe Li Yuteng Weng Dandan Bai Yanping Jia Yingdong Liu Yalin Zhang Xiaochen Kou Yanhong Zhao Jingling Ruan Jiayu Chen Jiqing Yin Hong Wang Xiaoming Teng Zuolin Wang Wenqiang Liu Shaorong Gao
author_facet	Yanhe Li Yuteng Weng Dandan Bai Yanping Jia Yingdong Liu Yalin Zhang Xiaochen Kou Yanhong Zhao Jingling Ruan Jiayu Chen Jiqing Yin Hong Wang Xiaoming Teng Zuolin Wang Wenqiang Liu Shaorong Gao
author_sort	Yanhe Li
title	Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
title_short	Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
title_full	Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
title_fullStr	Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
title_full_unstemmed	Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
title_sort	precise allele-specific genome editing by spatiotemporal control of crispr-cas9 via pronuclear transplantation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/cc16067a91c84bc8a8ce6c62671c27c7
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Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

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