Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.

Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discr...

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Autores principales:	William A Braff, Dana Willner, Philip Hugenholtz, Korneel Rabaey, Cullen R Buie
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce210cb2b9c14d65817b9f095f325c4e
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spelling	oai:doaj.org-article:ce210cb2b9c14d65817b9f095f325c4e2021-11-18T08:50:46ZDielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.1932-620310.1371/journal.pone.0076751https://doaj.org/article/ce210cb2b9c14d65817b9f095f325c4e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146923/?tool=EBIhttps://doaj.org/toc/1932-6203Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.William A BraffDana WillnerPhilip HugenholtzKorneel RabaeyCullen R BuiePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76751 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q William A Braff Dana Willner Philip Hugenholtz Korneel Rabaey Cullen R Buie Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
description	Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.
format	article
author	William A Braff Dana Willner Philip Hugenholtz Korneel Rabaey Cullen R Buie
author_facet	William A Braff Dana Willner Philip Hugenholtz Korneel Rabaey Cullen R Buie
author_sort	William A Braff
title	Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
title_short	Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
title_full	Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
title_fullStr	Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
title_full_unstemmed	Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
title_sort	dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ce210cb2b9c14d65817b9f095f325c4e
work_keys_str_mv	AT williamabraff dielectrophoresisbaseddiscriminationofbacteriaatthestrainlevelbasedontheirsurfaceproperties AT danawillner dielectrophoresisbaseddiscriminationofbacteriaatthestrainlevelbasedontheirsurfaceproperties AT philiphugenholtz dielectrophoresisbaseddiscriminationofbacteriaatthestrainlevelbasedontheirsurfaceproperties AT korneelrabaey dielectrophoresisbaseddiscriminationofbacteriaatthestrainlevelbasedontheirsurfaceproperties AT cullenrbuie dielectrophoresisbaseddiscriminationofbacteriaatthestrainlevelbasedontheirsurfaceproperties
_version_	1718421288500854784

Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.

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