An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue

Abstract Biolistic delivery is widely used for genetic transformation but inconsistency between bombardment samples for transient gene expression analysis often hinders quantitative analyses. We developed a methodology to improve the consistency of biolistic delivery results by using a double-barrel...

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Autores principales: Kyle Miller, Alan L. Eggenberger, Keunsub Lee, Fei Liu, Minjeong Kang, Madison Drent, Andrew Ruba, Tyler Kirscht, Kan Wang, Shan Jiang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/cf7ad386493a4ad5925bfe5e9dd79613
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