The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states

Summary: Heterochromatin formation requires three distinct steps: nucleation, self-propagation (spreading) along the chromosome, and faithful maintenance after each replication cycle. Impeding any of those steps induces heterochromatin defects and improper gene expression. The essential histone chap...

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Autores principales:	Magdalena Murawska, R.A. Greenstein, Tamas Schauer, Karl C.F. Olsen, Henry Ng, Andreas G. Ladurner, Bassem Al-Sady, Sigurd Braun
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	FACT histone chaperone heterochromatin spreading Epe1 histone turnover Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d053a21db4ae458c81001d81be4d5d4d
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spelling	oai:doaj.org-article:d053a21db4ae458c81001d81be4d5d4d2021-11-04T04:29:40ZThe histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states2211-124710.1016/j.celrep.2021.109944https://doaj.org/article/d053a21db4ae458c81001d81be4d5d4d2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721014170https://doaj.org/toc/2211-1247Summary: Heterochromatin formation requires three distinct steps: nucleation, self-propagation (spreading) along the chromosome, and faithful maintenance after each replication cycle. Impeding any of those steps induces heterochromatin defects and improper gene expression. The essential histone chaperone FACT (facilitates chromatin transcription) has been implicated in heterochromatin silencing, but the mechanisms by which FACT engages in this process remain opaque. Here, we pinpoint its function to the heterochromatin spreading process in fission yeast. FACT impairment reduces nucleation-distal H3K9me3 and HP1/Swi6 accumulation at subtelomeres and derepresses genes in the vicinity of heterochromatin boundaries. FACT promotes spreading by repressing heterochromatic histone turnover, which is crucial for the H3K9me2 to me3 transition that enables spreading. FACT mutant spreading defects are suppressed by removal of the H3K9 methylation antagonist Epe1. Together, our study identifies FACT as a histone chaperone that promotes heterochromatin spreading and lends support to the model that regulated histone turnover controls the propagation of repressive methylation marks.Magdalena MurawskaR.A. GreensteinTamas SchauerKarl C.F. OlsenHenry NgAndreas G. LadurnerBassem Al-SadySigurd BraunElsevierarticleFACThistone chaperoneheterochromatin spreadingEpe1histone turnoverBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 5, Pp 109944- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FACT histone chaperone heterochromatin spreading Epe1 histone turnover Biology (General) QH301-705.5
spellingShingle	FACT histone chaperone heterochromatin spreading Epe1 histone turnover Biology (General) QH301-705.5 Magdalena Murawska R.A. Greenstein Tamas Schauer Karl C.F. Olsen Henry Ng Andreas G. Ladurner Bassem Al-Sady Sigurd Braun The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states
description	Summary: Heterochromatin formation requires three distinct steps: nucleation, self-propagation (spreading) along the chromosome, and faithful maintenance after each replication cycle. Impeding any of those steps induces heterochromatin defects and improper gene expression. The essential histone chaperone FACT (facilitates chromatin transcription) has been implicated in heterochromatin silencing, but the mechanisms by which FACT engages in this process remain opaque. Here, we pinpoint its function to the heterochromatin spreading process in fission yeast. FACT impairment reduces nucleation-distal H3K9me3 and HP1/Swi6 accumulation at subtelomeres and derepresses genes in the vicinity of heterochromatin boundaries. FACT promotes spreading by repressing heterochromatic histone turnover, which is crucial for the H3K9me2 to me3 transition that enables spreading. FACT mutant spreading defects are suppressed by removal of the H3K9 methylation antagonist Epe1. Together, our study identifies FACT as a histone chaperone that promotes heterochromatin spreading and lends support to the model that regulated histone turnover controls the propagation of repressive methylation marks.
format	article
author	Magdalena Murawska R.A. Greenstein Tamas Schauer Karl C.F. Olsen Henry Ng Andreas G. Ladurner Bassem Al-Sady Sigurd Braun
author_facet	Magdalena Murawska R.A. Greenstein Tamas Schauer Karl C.F. Olsen Henry Ng Andreas G. Ladurner Bassem Al-Sady Sigurd Braun
author_sort	Magdalena Murawska
title	The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states
title_short	The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states
title_full	The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states
title_fullStr	The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states
title_full_unstemmed	The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states
title_sort	histone chaperone fact facilitates heterochromatin spreading by regulating histone turnover and h3k9 methylation states
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d053a21db4ae458c81001d81be4d5d4d
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The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states

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