Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder

Abstract We used 16S ribosomal RNA sequencing to evaluate changes in the gut microbiota of mice fed a diet supplemented with either raw or cooked beef loin powder for 9 weeks. Male BALB/c mice (n = 60) were randomly allocated to five groups: mice fed AIN-93G chow (CON), chow containing 5% (5RB) and...

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Autores principales:	Hye-Jin Kim, Dongwook Kim, Kwan-Woo Kim, Sang-Hoon Lee, Aera Jang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0ca317ad3c64011833ad8cc41a4ba45
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spelling	oai:doaj.org-article:d0ca317ad3c64011833ad8cc41a4ba452021-12-02T17:51:06ZComparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder10.1038/s41598-021-90461-72045-2322https://doaj.org/article/d0ca317ad3c64011833ad8cc41a4ba452021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90461-7https://doaj.org/toc/2045-2322Abstract We used 16S ribosomal RNA sequencing to evaluate changes in the gut microbiota of mice fed a diet supplemented with either raw or cooked beef loin powder for 9 weeks. Male BALB/c mice (n = 60) were randomly allocated to five groups: mice fed AIN-93G chow (CON), chow containing 5% (5RB) and 10% (10RB) raw beef loin powder, and chow containing 5% (5CB) and 10% (10CB) cooked beef loin powder. Dietary supplementation with both RB and CB increased the relative abundance of Clostridiales compared to the CON diet (p < 0.05). Mice fed 10RB showed a significantly higher relative abundance of Firmicutes (p = 0.018) and Lactobacillus (p = 0.001) than CON mice, and the ratio of Firmicutes/Bacteroidetes showed an increasing trend in the 10RB mice (p > 0.05). Mice fed 10CB showed a higher abundance of Peptostreptococcaceae and a lower abundance of Desulfovibrionaceae compared with the CON mice (p < 0.05). Genes for glycan biosynthesis, which result in short-chain fatty acid synthesis, were enriched in the CB mice compared to the RB mice, which was correlated to a high abundance of Bacteroides. Overall, dietary RB and CB changed the gut microbiota of mice (p < 0.05).Hye-Jin KimDongwook KimKwan-Woo KimSang-Hoon LeeAera JangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hye-Jin Kim Dongwook Kim Kwan-Woo Kim Sang-Hoon Lee Aera Jang Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
description	Abstract We used 16S ribosomal RNA sequencing to evaluate changes in the gut microbiota of mice fed a diet supplemented with either raw or cooked beef loin powder for 9 weeks. Male BALB/c mice (n = 60) were randomly allocated to five groups: mice fed AIN-93G chow (CON), chow containing 5% (5RB) and 10% (10RB) raw beef loin powder, and chow containing 5% (5CB) and 10% (10CB) cooked beef loin powder. Dietary supplementation with both RB and CB increased the relative abundance of Clostridiales compared to the CON diet (p < 0.05). Mice fed 10RB showed a significantly higher relative abundance of Firmicutes (p = 0.018) and Lactobacillus (p = 0.001) than CON mice, and the ratio of Firmicutes/Bacteroidetes showed an increasing trend in the 10RB mice (p > 0.05). Mice fed 10CB showed a higher abundance of Peptostreptococcaceae and a lower abundance of Desulfovibrionaceae compared with the CON mice (p < 0.05). Genes for glycan biosynthesis, which result in short-chain fatty acid synthesis, were enriched in the CB mice compared to the RB mice, which was correlated to a high abundance of Bacteroides. Overall, dietary RB and CB changed the gut microbiota of mice (p < 0.05).
format	article
author	Hye-Jin Kim Dongwook Kim Kwan-Woo Kim Sang-Hoon Lee Aera Jang
author_facet	Hye-Jin Kim Dongwook Kim Kwan-Woo Kim Sang-Hoon Lee Aera Jang
author_sort	Hye-Jin Kim
title	Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
title_short	Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
title_full	Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
title_fullStr	Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
title_full_unstemmed	Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
title_sort	comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0ca317ad3c64011833ad8cc41a4ba45
work_keys_str_mv	AT hyejinkim comparativeanalysisofthegutmicrobiotaofmicefedadietsupplementedwithrawandcookedbeefloinpowder AT dongwookkim comparativeanalysisofthegutmicrobiotaofmicefedadietsupplementedwithrawandcookedbeefloinpowder AT kwanwookim comparativeanalysisofthegutmicrobiotaofmicefedadietsupplementedwithrawandcookedbeefloinpowder AT sanghoonlee comparativeanalysisofthegutmicrobiotaofmicefedadietsupplementedwithrawandcookedbeefloinpowder AT aerajang comparativeanalysisofthegutmicrobiotaofmicefedadietsupplementedwithrawandcookedbeefloinpowder
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Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder

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