Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast

Modern high-throughput screening is a versatile method used widely in academic and industrial labs for genetic and pharmacological testing, but is underutilized in undergraduate laboratories. We designed an open inquiry-based laboratory activity exploiting a small-scale high-throughput screening ass...

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Auteurs principaux:	Veronica A. Segarra, Carrie Wilson, Kelley Lowery, Slade Ransdell, Jamie Schnuck, Paul Way, Melissa C. Srougi
Format:	article
Langue:	EN
Publié:	American Society for Microbiology 2018
Sujets:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/d1c8b90053b74f129963059fa1b7910f
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spelling	oai:doaj.org-article:d1c8b90053b74f129963059fa1b7910f2021-11-15T15:04:11ZStudent-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast10.1128/jmbe.v19i2.15421935-78851935-7877https://doaj.org/article/d1c8b90053b74f129963059fa1b7910f2018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i2.1542https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Modern high-throughput screening is a versatile method used widely in academic and industrial labs for genetic and pharmacological testing, but is underutilized in undergraduate laboratories. We designed an open inquiry-based laboratory activity exploiting a small-scale high-throughput screening assay of the budding yeast S. cerevisiae to promote active learning and introduce students to modern data collection and analysis techniques. In this activity, students at both the high school and university levels generate growth curves of S. cerevisiae using a microplate reader after treatment with their insult of choice. This lab presents an ideal opportunity for both high school and university students to actively learn about the process of science as well as high-throughput data collection and analysis in a cost-effective manner.Veronica A. SegarraCarrie WilsonKelley LowerySlade RansdellJamie SchnuckPaul WayMelissa C. SrougiAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Veronica A. Segarra Carrie Wilson Kelley Lowery Slade Ransdell Jamie Schnuck Paul Way Melissa C. Srougi Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast
description	Modern high-throughput screening is a versatile method used widely in academic and industrial labs for genetic and pharmacological testing, but is underutilized in undergraduate laboratories. We designed an open inquiry-based laboratory activity exploiting a small-scale high-throughput screening assay of the budding yeast S. cerevisiae to promote active learning and introduce students to modern data collection and analysis techniques. In this activity, students at both the high school and university levels generate growth curves of S. cerevisiae using a microplate reader after treatment with their insult of choice. This lab presents an ideal opportunity for both high school and university students to actively learn about the process of science as well as high-throughput data collection and analysis in a cost-effective manner.
format	article
author	Veronica A. Segarra Carrie Wilson Kelley Lowery Slade Ransdell Jamie Schnuck Paul Way Melissa C. Srougi
author_facet	Veronica A. Segarra Carrie Wilson Kelley Lowery Slade Ransdell Jamie Schnuck Paul Way Melissa C. Srougi
author_sort	Veronica A. Segarra
title	Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast
title_short	Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast
title_full	Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast
title_fullStr	Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast
title_full_unstemmed	Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast
title_sort	student-designed high-throughput assays to assess effects of growth insults in budding yeast
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/d1c8b90053b74f129963059fa1b7910f
work_keys_str_mv	AT veronicaasegarra studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast AT carriewilson studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast AT kelleylowery studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast AT sladeransdell studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast AT jamieschnuck studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast AT paulway studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast AT melissacsrougi studentdesignedhighthroughputassaystoassesseffectsofgrowthinsultsinbuddingyeast
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Student-Designed High-Throughput Assays to Assess Effects of Growth Insults in Budding Yeast

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