Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro

Backbone extended monomers are poorly compatible with the natural ribosomes, impeding their polymerization into polypeptides. Here the authors design non-canonical amino acid analogs with cyclic structures or extended carbon chains and used an engineered ribosome to improve tRNA-charging and incorpo...

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Auteurs principaux: Joongoo Lee, Kevin J. Schwarz, Do Soon Kim, Jeffrey S. Moore, Michael C. Jewett
Format: article
Langue:EN
Publié: Nature Portfolio 2020
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Accès en ligne:https://doaj.org/article/d1f0310e1e4a4c63ab01552ba5fb1c77
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