An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.
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Auteurs principaux: | , , |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2020
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Sujets: | |
Accès en ligne: | https://doaj.org/article/d32db6de3fea472f85940d2aac88783f |
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