An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.

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Auteurs principaux: Filipe Pinto, Ella Lucille Thornton, Baojun Wang
Format: article
Langue:EN
Publié: Nature Portfolio 2020
Sujets:
Q
Accès en ligne:https://doaj.org/article/d32db6de3fea472f85940d2aac88783f
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