An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.

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Detalles Bibliográficos
Autores principales:	Filipe Pinto, Ella Lucille Thornton, Baojun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d32db6de3fea472f85940d2aac88783f
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id	oai:doaj.org-article:d32db6de3fea472f85940d2aac88783f
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spelling	oai:doaj.org-article:d32db6de3fea472f85940d2aac88783f2021-12-02T14:40:30ZAn expanded library of orthogonal split inteins enables modular multi-peptide assemblies10.1038/s41467-020-15272-22041-1723https://doaj.org/article/d32db6de3fea472f85940d2aac88783f2020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15272-2https://doaj.org/toc/2041-1723Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.Filipe PintoElla Lucille ThorntonBaojun WangNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Filipe Pinto Ella Lucille Thornton Baojun Wang An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
description	Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.
format	article
author	Filipe Pinto Ella Lucille Thornton Baojun Wang
author_facet	Filipe Pinto Ella Lucille Thornton Baojun Wang
author_sort	Filipe Pinto
title	An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
title_short	An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
title_full	An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
title_fullStr	An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
title_full_unstemmed	An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
title_sort	expanded library of orthogonal split inteins enables modular multi-peptide assemblies
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d32db6de3fea472f85940d2aac88783f
work_keys_str_mv	AT filipepinto anexpandedlibraryoforthogonalsplitinteinsenablesmodularmultipeptideassemblies AT ellalucillethornton anexpandedlibraryoforthogonalsplitinteinsenablesmodularmultipeptideassemblies AT baojunwang anexpandedlibraryoforthogonalsplitinteinsenablesmodularmultipeptideassemblies AT filipepinto expandedlibraryoforthogonalsplitinteinsenablesmodularmultipeptideassemblies AT ellalucillethornton expandedlibraryoforthogonalsplitinteinsenablesmodularmultipeptideassemblies AT baojunwang expandedlibraryoforthogonalsplitinteinsenablesmodularmultipeptideassemblies
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An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

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