A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
Abstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the f...
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Nature Portfolio
2021
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oai:doaj.org-article:d3e61c501c474ee4bce5627f7aeb90672021-12-02T17:39:45ZA cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells10.1038/s41541-021-00346-z2059-0105https://doaj.org/article/d3e61c501c474ee4bce5627f7aeb90672021-06-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00346-zhttps://doaj.org/toc/2059-0105Abstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.Simon G. P. FunnellBabak AfroughJohn James BaczenasNeil BerryKevin R. BewleyRebecca BradfordClint FlorenceYann Le DuffMark LewisRyan V. MoriartyShelby L. O. ConnorKaren L. OsmanSteven PullanSujatha RashidKevin S. RichardsKimberly J. StempleIvana KnezevicNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-5 (2021) |
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DOAJ |
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Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Simon G. P. Funnell Babak Afrough John James Baczenas Neil Berry Kevin R. Bewley Rebecca Bradford Clint Florence Yann Le Duff Mark Lewis Ryan V. Moriarty Shelby L. O. Connor Karen L. Osman Steven Pullan Sujatha Rashid Kevin S. Richards Kimberly J. Stemple Ivana Knezevic A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| description |
Abstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells. |
| format |
article |
| author |
Simon G. P. Funnell Babak Afrough John James Baczenas Neil Berry Kevin R. Bewley Rebecca Bradford Clint Florence Yann Le Duff Mark Lewis Ryan V. Moriarty Shelby L. O. Connor Karen L. Osman Steven Pullan Sujatha Rashid Kevin S. Richards Kimberly J. Stemple Ivana Knezevic |
| author_facet |
Simon G. P. Funnell Babak Afrough John James Baczenas Neil Berry Kevin R. Bewley Rebecca Bradford Clint Florence Yann Le Duff Mark Lewis Ryan V. Moriarty Shelby L. O. Connor Karen L. Osman Steven Pullan Sujatha Rashid Kevin S. Richards Kimberly J. Stemple Ivana Knezevic |
| author_sort |
Simon G. P. Funnell |
| title |
A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_short |
A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_full |
A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_fullStr |
A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_full_unstemmed |
A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_sort |
cautionary perspective regarding the isolation and serial propagation of sars-cov-2 in vero cells |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/d3e61c501c474ee4bce5627f7aeb9067 |
| work_keys_str_mv |
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