Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.

Polycomb group (PcG) proteins are transcriptional repressors of genes involved in development and differentiation, and also maintain repression of key genes involved in the cell cycle, indirectly regulating cell proliferation. The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein...

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Autores principales:	Emilio Lecona, Luis Alejandro Rojas, Roberto Bonasio, Andrew Johnston, Oscar Fernández-Capetillo, Danny Reinberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d4ddbaaa48fc4b02b8abd870469d19f8
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spelling	oai:doaj.org-article:d4ddbaaa48fc4b02b8abd870469d19f82021-11-18T05:37:41ZPolycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.1544-91731545-788510.1371/journal.pbio.1001737https://doaj.org/article/d4ddbaaa48fc4b02b8abd870469d19f82013-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358021/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Polycomb group (PcG) proteins are transcriptional repressors of genes involved in development and differentiation, and also maintain repression of key genes involved in the cell cycle, indirectly regulating cell proliferation. The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic. Here, we purified SCML2B and found that it forms a stable complex with CDK/CYCLIN/p21 and p27, enhancing the inhibitory effect of p21/p27. SCML2B participates in the G1/S checkpoint by stabilizing p21 and favoring its interaction with CDK2/CYCE, resulting in decreased kinase activity and inhibited progression through G1. In turn, CDK/CYCLIN complexes phosphorylate SCML2, and the interaction of SCML2B with CDK2 is regulated through the cell cycle. These findings highlight a direct crosstalk between the Polycomb system of cellular memory and the cell-cycle machinery in mammals.Emilio LeconaLuis Alejandro RojasRoberto BonasioAndrew JohnstonOscar Fernández-CapetilloDanny ReinbergPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 12, p e1001737 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Emilio Lecona Luis Alejandro Rojas Roberto Bonasio Andrew Johnston Oscar Fernández-Capetillo Danny Reinberg Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.
description	Polycomb group (PcG) proteins are transcriptional repressors of genes involved in development and differentiation, and also maintain repression of key genes involved in the cell cycle, indirectly regulating cell proliferation. The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic. Here, we purified SCML2B and found that it forms a stable complex with CDK/CYCLIN/p21 and p27, enhancing the inhibitory effect of p21/p27. SCML2B participates in the G1/S checkpoint by stabilizing p21 and favoring its interaction with CDK2/CYCE, resulting in decreased kinase activity and inhibited progression through G1. In turn, CDK/CYCLIN complexes phosphorylate SCML2, and the interaction of SCML2B with CDK2 is regulated through the cell cycle. These findings highlight a direct crosstalk between the Polycomb system of cellular memory and the cell-cycle machinery in mammals.
format	article
author	Emilio Lecona Luis Alejandro Rojas Roberto Bonasio Andrew Johnston Oscar Fernández-Capetillo Danny Reinberg
author_facet	Emilio Lecona Luis Alejandro Rojas Roberto Bonasio Andrew Johnston Oscar Fernández-Capetillo Danny Reinberg
author_sort	Emilio Lecona
title	Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.
title_short	Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.
title_full	Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.
title_fullStr	Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.
title_full_unstemmed	Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.
title_sort	polycomb protein scml2 regulates the cell cycle by binding and modulating cdk/cyclin/p21 complexes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d4ddbaaa48fc4b02b8abd870469d19f8
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Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.

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