Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks

Embryonic thermal programming has been shown to improve foie gras production in overfed mule ducks. However, the mechanisms at the origin of this programming have not yet been characterized. In this study, we investigated the effect of embryonic thermal manipulation (+1°C, 16 h/24 h from embryonic (...

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Autores principales:	William Massimino, Charlotte Andrieux, Sandra Biasutti, Stéphane Davail, Marie-Dominique Bernadet, Tracy Pioche, Karine Ricaud, Karine Gontier, Mireille Morisson, Anne Collin, Stéphane Panserat, Marianne Houssier
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	embryonic thermal programming liver metabolism gene expression duck Physiology QP1-981
Acceso en línea:	https://doaj.org/article/d51ab679c74a43a6a5df87970ed78c56
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spelling	oai:doaj.org-article:d51ab679c74a43a6a5df87970ed78c562021-12-03T06:46:42ZImpacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks1664-042X10.3389/fphys.2021.779689https://doaj.org/article/d51ab679c74a43a6a5df87970ed78c562021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.779689/fullhttps://doaj.org/toc/1664-042XEmbryonic thermal programming has been shown to improve foie gras production in overfed mule ducks. However, the mechanisms at the origin of this programming have not yet been characterized. In this study, we investigated the effect of embryonic thermal manipulation (+1°C, 16 h/24 h from embryonic (E) day 13 to E27) on the hepatic expression of genes involved in lipid and carbohydrate metabolisms, stress, cell proliferation and thyroid hormone pathways at the end of thermal manipulation and before and after overfeeding (OF) in mule ducks. Gene expression analyses were performed by classic or high throughput real-time qPCR. First, we confirmed well-known results with strong impact of OF on the expression of genes involved in lipid and carbohydrates metabolisms. Then we observed an impact of OF on the hepatic expression of genes involved in the thyroid pathway, stress and cell proliferation. Only a small number of genes showed modulation of expression related to thermal programming at the time of OF, and only one was also impacted at the end of the thermal manipulation. For the first time, we explored the molecular mechanisms of embryonic thermal programming from the end of heat treatment to the programmed adult phenotype with optimized liver metabolism.William MassiminoCharlotte AndrieuxSandra BiasuttiStéphane DavailMarie-Dominique BernadetTracy PiocheKarine RicaudKarine GontierMireille MorissonAnne CollinStéphane PanseratMarianne HoussierFrontiers Media S.A.articleembryonic thermal programminglivermetabolismgene expressionduckPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	embryonic thermal programming liver metabolism gene expression duck Physiology QP1-981
spellingShingle	embryonic thermal programming liver metabolism gene expression duck Physiology QP1-981 William Massimino Charlotte Andrieux Sandra Biasutti Stéphane Davail Marie-Dominique Bernadet Tracy Pioche Karine Ricaud Karine Gontier Mireille Morisson Anne Collin Stéphane Panserat Marianne Houssier Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks
description	Embryonic thermal programming has been shown to improve foie gras production in overfed mule ducks. However, the mechanisms at the origin of this programming have not yet been characterized. In this study, we investigated the effect of embryonic thermal manipulation (+1°C, 16 h/24 h from embryonic (E) day 13 to E27) on the hepatic expression of genes involved in lipid and carbohydrate metabolisms, stress, cell proliferation and thyroid hormone pathways at the end of thermal manipulation and before and after overfeeding (OF) in mule ducks. Gene expression analyses were performed by classic or high throughput real-time qPCR. First, we confirmed well-known results with strong impact of OF on the expression of genes involved in lipid and carbohydrates metabolisms. Then we observed an impact of OF on the hepatic expression of genes involved in the thyroid pathway, stress and cell proliferation. Only a small number of genes showed modulation of expression related to thermal programming at the time of OF, and only one was also impacted at the end of the thermal manipulation. For the first time, we explored the molecular mechanisms of embryonic thermal programming from the end of heat treatment to the programmed adult phenotype with optimized liver metabolism.
format	article
author	William Massimino Charlotte Andrieux Sandra Biasutti Stéphane Davail Marie-Dominique Bernadet Tracy Pioche Karine Ricaud Karine Gontier Mireille Morisson Anne Collin Stéphane Panserat Marianne Houssier
author_facet	William Massimino Charlotte Andrieux Sandra Biasutti Stéphane Davail Marie-Dominique Bernadet Tracy Pioche Karine Ricaud Karine Gontier Mireille Morisson Anne Collin Stéphane Panserat Marianne Houssier
author_sort	William Massimino
title	Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks
title_short	Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks
title_full	Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks
title_fullStr	Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks
title_full_unstemmed	Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks
title_sort	impacts of embryonic thermal programming on the expression of genes involved in foie gras production in mule ducks
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d51ab679c74a43a6a5df87970ed78c56
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Impacts of Embryonic Thermal Programming on the Expression of Genes Involved in Foie gras Production in Mule Ducks

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