Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress
This study evaluated epigallocatechin gallate’s (EGCG’s, 400 mg/kg) effect on meat quality and muscle antioxidant status of broilers under acute heat stress (AHS). A total of 144 21-day-old male Huainan partridge chickens were randomly allocated to the EGCG-free group (12 replicates) and the EGCG gr...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/d5b83b10c68345548f4c0dbd11f73802 |
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| Sumario: | This study evaluated epigallocatechin gallate’s (EGCG’s, 400 mg/kg) effect on meat quality and muscle antioxidant status of broilers under acute heat stress (AHS). A total of 144 21-day-old male Huainan partridge chickens were randomly allocated to the EGCG-free group (12 replicates) and the EGCG group (6 replicates). On day 94, the EGCG-free group was divided into the control group (CON) and the AHS group, and then AHS group and EGCG group (identified as AHS + EGCG group) were treated with AHS (33 ± 1 °C for 12 h). AHS increased (<i>p</i> < 0.05) L*<sub>24h</sub>, drip loss, muscle lactic acid, malondialdehyde (MDA) contents, and kelch-like ECH-associated protein 1 (<i>Keap1</i>) mRNA level, and decreased (<i>p</i> < 0.05) eviscerated percentage, pH<sub>24h</sub>, a*, muscle total superoxide dismutase (T-SOD) activity, the ratio of T-SOD/MDA and glutathione peroxidase /MDA, glycogen content, and nuclear factor erythroid 2-related 2 (<i>Nrf2</i>), catalase (<i>CAT</i>), NAD(P)H/quinone dehydrogenase 1 (<i>NQO1</i>) mRNA levels. The AHS + EGCG group exhibited lower (<i>p</i> < 0.05) L*<sub>24h</sub>, drip loss, muscle lactic acid, MDA contents and <i>Keap1</i> mRNA level, and greater (<i>p</i> < 0.05) eviscerated percentage, pH<sub>24h</sub>, a*, muscle T-SOD activity, the ratio of T-SOD/MDA, <i>Nrf2</i>, and <i>NQO1</i> mRNA levels compared with the AHS group. In conclusion, EGCG protects against AHS-impaired meat quality by improving muscle antioxidant capacity, which seems to be associated with the activation of the <i>Nrf2</i> signaling pathway. |
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