Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress
This study evaluated epigallocatechin gallate’s (EGCG’s, 400 mg/kg) effect on meat quality and muscle antioxidant status of broilers under acute heat stress (AHS). A total of 144 21-day-old male Huainan partridge chickens were randomly allocated to the EGCG-free group (12 replicates) and the EGCG gr...
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oai:doaj.org-article:d5b83b10c68345548f4c0dbd11f738022021-11-25T16:20:46ZEffects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress10.3390/ani111132962076-2615https://doaj.org/article/d5b83b10c68345548f4c0dbd11f738022021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3296https://doaj.org/toc/2076-2615This study evaluated epigallocatechin gallate’s (EGCG’s, 400 mg/kg) effect on meat quality and muscle antioxidant status of broilers under acute heat stress (AHS). A total of 144 21-day-old male Huainan partridge chickens were randomly allocated to the EGCG-free group (12 replicates) and the EGCG group (6 replicates). On day 94, the EGCG-free group was divided into the control group (CON) and the AHS group, and then AHS group and EGCG group (identified as AHS + EGCG group) were treated with AHS (33 ± 1 °C for 12 h). AHS increased (<i>p</i> < 0.05) L*<sub>24h</sub>, drip loss, muscle lactic acid, malondialdehyde (MDA) contents, and kelch-like ECH-associated protein 1 (<i>Keap1</i>) mRNA level, and decreased (<i>p</i> < 0.05) eviscerated percentage, pH<sub>24h</sub>, a*, muscle total superoxide dismutase (T-SOD) activity, the ratio of T-SOD/MDA and glutathione peroxidase /MDA, glycogen content, and nuclear factor erythroid 2-related 2 (<i>Nrf2</i>), catalase (<i>CAT</i>), NAD(P)H/quinone dehydrogenase 1 (<i>NQO1</i>) mRNA levels. The AHS + EGCG group exhibited lower (<i>p</i> < 0.05) L*<sub>24h</sub>, drip loss, muscle lactic acid, MDA contents and <i>Keap1</i> mRNA level, and greater (<i>p</i> < 0.05) eviscerated percentage, pH<sub>24h</sub>, a*, muscle T-SOD activity, the ratio of T-SOD/MDA, <i>Nrf2</i>, and <i>NQO1</i> mRNA levels compared with the AHS group. In conclusion, EGCG protects against AHS-impaired meat quality by improving muscle antioxidant capacity, which seems to be associated with the activation of the <i>Nrf2</i> signaling pathway.Fei ZhaoXiaocheng WangYang LiXingyong ChenZhaoyu GengCheng ZhangMDPI AGarticleacute heat stressepigallocatechin gallatemeat qualityantioxidant capacitybroilerVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3296, p 3296 (2021) |
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acute heat stress epigallocatechin gallate meat quality antioxidant capacity broiler Veterinary medicine SF600-1100 Zoology QL1-991 |
| spellingShingle |
acute heat stress epigallocatechin gallate meat quality antioxidant capacity broiler Veterinary medicine SF600-1100 Zoology QL1-991 Fei Zhao Xiaocheng Wang Yang Li Xingyong Chen Zhaoyu Geng Cheng Zhang Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress |
| description |
This study evaluated epigallocatechin gallate’s (EGCG’s, 400 mg/kg) effect on meat quality and muscle antioxidant status of broilers under acute heat stress (AHS). A total of 144 21-day-old male Huainan partridge chickens were randomly allocated to the EGCG-free group (12 replicates) and the EGCG group (6 replicates). On day 94, the EGCG-free group was divided into the control group (CON) and the AHS group, and then AHS group and EGCG group (identified as AHS + EGCG group) were treated with AHS (33 ± 1 °C for 12 h). AHS increased (<i>p</i> < 0.05) L*<sub>24h</sub>, drip loss, muscle lactic acid, malondialdehyde (MDA) contents, and kelch-like ECH-associated protein 1 (<i>Keap1</i>) mRNA level, and decreased (<i>p</i> < 0.05) eviscerated percentage, pH<sub>24h</sub>, a*, muscle total superoxide dismutase (T-SOD) activity, the ratio of T-SOD/MDA and glutathione peroxidase /MDA, glycogen content, and nuclear factor erythroid 2-related 2 (<i>Nrf2</i>), catalase (<i>CAT</i>), NAD(P)H/quinone dehydrogenase 1 (<i>NQO1</i>) mRNA levels. The AHS + EGCG group exhibited lower (<i>p</i> < 0.05) L*<sub>24h</sub>, drip loss, muscle lactic acid, MDA contents and <i>Keap1</i> mRNA level, and greater (<i>p</i> < 0.05) eviscerated percentage, pH<sub>24h</sub>, a*, muscle T-SOD activity, the ratio of T-SOD/MDA, <i>Nrf2</i>, and <i>NQO1</i> mRNA levels compared with the AHS group. In conclusion, EGCG protects against AHS-impaired meat quality by improving muscle antioxidant capacity, which seems to be associated with the activation of the <i>Nrf2</i> signaling pathway. |
| format |
article |
| author |
Fei Zhao Xiaocheng Wang Yang Li Xingyong Chen Zhaoyu Geng Cheng Zhang |
| author_facet |
Fei Zhao Xiaocheng Wang Yang Li Xingyong Chen Zhaoyu Geng Cheng Zhang |
| author_sort |
Fei Zhao |
| title |
Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress |
| title_short |
Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress |
| title_full |
Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress |
| title_fullStr |
Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress |
| title_full_unstemmed |
Effects of Dietary Supplementation with Epigallocatechin Gallate on Meat Quality and Muscle Antioxidant Capacity of Broilers Subjected to Acute Heat Stress |
| title_sort |
effects of dietary supplementation with epigallocatechin gallate on meat quality and muscle antioxidant capacity of broilers subjected to acute heat stress |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/d5b83b10c68345548f4c0dbd11f73802 |
| work_keys_str_mv |
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| _version_ |
1718413229068124160 |