Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

Abstract Successful establishment of CRISPR/Cas9 genome editing technology in Plasmodium spp. has provided a powerful tool to transform Plasmodium falciparum into a genetically more tractable organism. Conditional gene regulation approaches are required to study the function of gene products critica...

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Bibliographic Details
Main Authors: Ellen Knuepfer, Marta Napiorkowska, Christiaan van Ooij, Anthony A. Holder
Format: article
Language:EN
Published: Nature Portfolio 2017
Subjects:
R
Q
Online Access:https://doaj.org/article/d5e83861321b4d35ab33c4b412310346
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