Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates

ABSTRACT In a previous mBio article, Wadsworth and colleagues (mBio 9:e01419-18, 2018, https://doi.org/10.1128/mBio.01419-18) described Neisseria gonorrhoeae isolates that express low levels of azithromycin (Azi) resistance. Whole-genome sequencing and bioinformatic analysis suggested that the isola...

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Auteur principal:	William M. Shafer
Format:	article
Langue:	EN
Publié:	American Society for Microbiology 2018
Sujets:	Neisseria gonorrhoeae antibiotic resistance gonorrhea transformation Microbiology QR1-502
Accès en ligne:	https://doaj.org/article/d6f8dcafebf64cdb8a4bd1608198e5f4
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spelling	oai:doaj.org-article:d6f8dcafebf64cdb8a4bd1608198e5f42021-11-15T15:58:21ZMosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates10.1128/mBio.01747-182150-7511https://doaj.org/article/d6f8dcafebf64cdb8a4bd1608198e5f42018-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01747-18https://doaj.org/toc/2150-7511ABSTRACT In a previous mBio article, Wadsworth and colleagues (mBio 9:e01419-18, 2018, https://doi.org/10.1128/mBio.01419-18) described Neisseria gonorrhoeae isolates that express low levels of azithromycin (Azi) resistance. Whole-genome sequencing and bioinformatic analysis suggested that the isolates had acquired DNA from commensal Neisseria spp. that caused numerous nucleotide changes in the mtr locus, which contains genes for a transcriptional repressor (MtrR) and three proteins (MtrC-MtrD-MtrE) that form a multidrug efflux pump known to export macrolides. Strong regions of linkage disequilibrium mapped to the overlapping mtrR and mtrCDE promoters and mtrD. Genetic analyses revealed that these mosaic-like sequences increased transcription of mtrCDE and MtrD function, respectively. These changes also had strong epistatic effects that collectively were responsible for decreased susceptibility to MtrCDE substrates, including Azi. The report emphasizes the importance of gene exchange among neisserial species and development of antibiotic resistance in gonococci, both of which have ramifications for detection of resistance markers and efficacy of antibiotic treatment regimens for gonorrhea.William M. ShaferAmerican Society for MicrobiologyarticleNeisseria gonorrhoeaeantibiotic resistancegonorrheatransformationMicrobiologyQR1-502ENmBio, Vol 9, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neisseria gonorrhoeae antibiotic resistance gonorrhea transformation Microbiology QR1-502
spellingShingle	Neisseria gonorrhoeae antibiotic resistance gonorrhea transformation Microbiology QR1-502 William M. Shafer Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates
description	ABSTRACT In a previous mBio article, Wadsworth and colleagues (mBio 9:e01419-18, 2018, https://doi.org/10.1128/mBio.01419-18) described Neisseria gonorrhoeae isolates that express low levels of azithromycin (Azi) resistance. Whole-genome sequencing and bioinformatic analysis suggested that the isolates had acquired DNA from commensal Neisseria spp. that caused numerous nucleotide changes in the mtr locus, which contains genes for a transcriptional repressor (MtrR) and three proteins (MtrC-MtrD-MtrE) that form a multidrug efflux pump known to export macrolides. Strong regions of linkage disequilibrium mapped to the overlapping mtrR and mtrCDE promoters and mtrD. Genetic analyses revealed that these mosaic-like sequences increased transcription of mtrCDE and MtrD function, respectively. These changes also had strong epistatic effects that collectively were responsible for decreased susceptibility to MtrCDE substrates, including Azi. The report emphasizes the importance of gene exchange among neisserial species and development of antibiotic resistance in gonococci, both of which have ramifications for detection of resistance markers and efficacy of antibiotic treatment regimens for gonorrhea.
format	article
author	William M. Shafer
author_facet	William M. Shafer
author_sort	William M. Shafer
title	Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates
title_short	Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates
title_full	Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates
title_fullStr	Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates
title_full_unstemmed	Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates
title_sort	mosaic drug efflux gene sequences from commensal <italic toggle="yes">neisseria</italic> can lead to low-level azithromycin resistance expressed by <italic toggle="yes">neisseria gonorrhoeae</italic> clinical isolates
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/d6f8dcafebf64cdb8a4bd1608198e5f4
work_keys_str_mv	AT williammshafer mosaicdrugeffluxgenesequencesfromcommensalitalictoggleyesneisseriaitaliccanleadtolowlevelazithromycinresistanceexpressedbyitalictoggleyesneisseriagonorrhoeaeitalicclinicalisolates
_version_	1718427057263738880

Mosaic Drug Efflux Gene Sequences from Commensal <italic toggle="yes">Neisseria</italic> Can Lead to Low-Level Azithromycin Resistance Expressed by <italic toggle="yes">Neisseria gonorrhoeae</italic> Clinical Isolates

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