Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Protein-protein interactions are fundamental to the regulation of protein activity and cellular phyisology. Here the authors present Split Intein-Mediated Protein Ligation, which uses bait and prey proteins fused to intein fragments to generate single intact proteins upon interaction.
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Auteurs principaux: | , , , , , , , , , , |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2020
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Accès en ligne: | https://doaj.org/article/d7c200701845452a9c8b0403041b6f6f |
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