Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition

Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition

Protein-protein interactions are fundamental to the regulation of protein activity and cellular phyisology. Here the authors present Split Intein-Mediated Protein Ligation, which uses bait and prey proteins fused to intein fragments to generate single intact proteins upon interaction.

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Detalles Bibliográficos
Autores principales: Zhong Yao, Farzaneh Aboualizadeh, Jason Kroll, Indira Akula, Jamie Snider, Anna Lyakisheva, Priscilla Tang, Max Kotlyar, Igor Jurisica, Mike Boxem, Igor Stagljar
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/d7c200701845452a9c8b0403041b6f6f
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