Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Metaboli engineering through gene overexpression, knock-down and knock-out is often carried out sequentially in a high labor, low-throughput manner. Here, the authors use CRISPR-mediated gene activation, interference and deletion to rapidly rewire S. cerevisiae metabolism in a single step.

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Bibliographic Details
Main Authors: Jiazhang Lian, Mohammad HamediRad, Sumeng Hu, Huimin Zhao
Format: article
Language:EN
Published: Nature Portfolio 2017
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Online Access:https://doaj.org/article/d8508d1d988448db9ca1fa3ed84b2c8d
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