Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Metaboli engineering through gene overexpression, knock-down and knock-out is often carried out sequentially in a high labor, low-throughput manner. Here, the authors use CRISPR-mediated gene activation, interference and deletion to rapidly rewire S. cerevisiae metabolism in a single step.

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiazhang Lian, Mohammad HamediRad, Sumeng Hu, Huimin Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d8508d1d988448db9ca1fa3ed84b2c8d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d8508d1d988448db9ca1fa3ed84b2c8d
record_format	dspace
spelling	oai:doaj.org-article:d8508d1d988448db9ca1fa3ed84b2c8d2021-12-02T14:40:37ZCombinatorial metabolic engineering using an orthogonal tri-functional CRISPR system10.1038/s41467-017-01695-x2041-1723https://doaj.org/article/d8508d1d988448db9ca1fa3ed84b2c8d2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01695-xhttps://doaj.org/toc/2041-1723Metaboli engineering through gene overexpression, knock-down and knock-out is often carried out sequentially in a high labor, low-throughput manner. Here, the authors use CRISPR-mediated gene activation, interference and deletion to rapidly rewire S. cerevisiae metabolism in a single step.Jiazhang LianMohammad HamediRadSumeng HuHuimin ZhaoNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jiazhang Lian Mohammad HamediRad Sumeng Hu Huimin Zhao Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
description	Metaboli engineering through gene overexpression, knock-down and knock-out is often carried out sequentially in a high labor, low-throughput manner. Here, the authors use CRISPR-mediated gene activation, interference and deletion to rapidly rewire S. cerevisiae metabolism in a single step.
format	article
author	Jiazhang Lian Mohammad HamediRad Sumeng Hu Huimin Zhao
author_facet	Jiazhang Lian Mohammad HamediRad Sumeng Hu Huimin Zhao
author_sort	Jiazhang Lian
title	Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
title_short	Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
title_full	Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
title_fullStr	Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
title_full_unstemmed	Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
title_sort	combinatorial metabolic engineering using an orthogonal tri-functional crispr system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d8508d1d988448db9ca1fa3ed84b2c8d
work_keys_str_mv	AT jiazhanglian combinatorialmetabolicengineeringusinganorthogonaltrifunctionalcrisprsystem AT mohammadhamedirad combinatorialmetabolicengineeringusinganorthogonaltrifunctionalcrisprsystem AT sumenghu combinatorialmetabolicengineeringusinganorthogonaltrifunctionalcrisprsystem AT huiminzhao combinatorialmetabolicengineeringusinganorthogonaltrifunctionalcrisprsystem
_version_	1718390266039107584

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Ejemplares similares