Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
Metaboli engineering through gene overexpression, knock-down and knock-out is often carried out sequentially in a high labor, low-throughput manner. Here, the authors use CRISPR-mediated gene activation, interference and deletion to rapidly rewire S. cerevisiae metabolism in a single step.
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Auteurs principaux: | Jiazhang Lian, Mohammad HamediRad, Sumeng Hu, Huimin Zhao |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2017
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Accès en ligne: | https://doaj.org/article/d8508d1d988448db9ca1fa3ed84b2c8d |
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