Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators

Rühl et al. report the generation of ratiometric genetically encoded voltage indicators (GEVIs) and establish that they can be used in high-throughput automated imaging to measure compound membrane potential (Vm) in mammalian cells. This method is implementable with standard fluorescence microscopes...

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Autores principales:	Philipp Rühl, Johanna M. Langner, Jasmin Reidel, Roland Schönherr, Toshinori Hoshi, Stefan H. Heinemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d8fdccc7299e4650ba56c7bdfa3b227d
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spelling	oai:doaj.org-article:d8fdccc7299e4650ba56c7bdfa3b227d2021-12-02T18:36:11ZMonitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators10.1038/s42003-021-02675-02399-3642https://doaj.org/article/d8fdccc7299e4650ba56c7bdfa3b227d2021-10-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02675-0https://doaj.org/toc/2399-3642Rühl et al. report the generation of ratiometric genetically encoded voltage indicators (GEVIs) and establish that they can be used in high-throughput automated imaging to measure compound membrane potential (Vm) in mammalian cells. This method is implementable with standard fluorescence microscopes and has the potential to offer insights into the role of the resting Vm in health and disease.Philipp RühlJohanna M. LangnerJasmin ReidelRoland SchönherrToshinori HoshiStefan H. HeinemannNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Philipp Rühl Johanna M. Langner Jasmin Reidel Roland Schönherr Toshinori Hoshi Stefan H. Heinemann Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
description	Rühl et al. report the generation of ratiometric genetically encoded voltage indicators (GEVIs) and establish that they can be used in high-throughput automated imaging to measure compound membrane potential (Vm) in mammalian cells. This method is implementable with standard fluorescence microscopes and has the potential to offer insights into the role of the resting Vm in health and disease.
format	article
author	Philipp Rühl Johanna M. Langner Jasmin Reidel Roland Schönherr Toshinori Hoshi Stefan H. Heinemann
author_facet	Philipp Rühl Johanna M. Langner Jasmin Reidel Roland Schönherr Toshinori Hoshi Stefan H. Heinemann
author_sort	Philipp Rühl
title	Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
title_short	Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
title_full	Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
title_fullStr	Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
title_full_unstemmed	Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
title_sort	monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d8fdccc7299e4650ba56c7bdfa3b227d
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Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators

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