Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy

Determining molecular clustering in Photoactivated Localization Microscopy (PALM) experiments requires knowledge of the blinking properties of the fluorophore to prevent overcounting artefacts. Here the authors develop an experimental and analytical framework to determine the blinking parameters of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: René Platzer, Benedikt K. Rossboth, Magdalena C. Schneider, Eva Sevcsik, Florian Baumgart, Hannes Stockinger, Gerhard J. Schütz, Johannes B. Huppa, Mario Brameshuber
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Q
Acceso en línea:https://doaj.org/article/d92f7a805d0a4aa697e8c1c2b9ffabca
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!