Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy

Determining molecular clustering in Photoactivated Localization Microscopy (PALM) experiments requires knowledge of the blinking properties of the fluorophore to prevent overcounting artefacts. Here the authors develop an experimental and analytical framework to determine the blinking parameters of...

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Autores principales:	René Platzer, Benedikt K. Rossboth, Magdalena C. Schneider, Eva Sevcsik, Florian Baumgart, Hannes Stockinger, Gerhard J. Schütz, Johannes B. Huppa, Mario Brameshuber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d92f7a805d0a4aa697e8c1c2b9ffabca
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spelling	oai:doaj.org-article:d92f7a805d0a4aa697e8c1c2b9ffabca2021-12-02T18:37:29ZUnscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy10.1038/s41467-020-18726-92041-1723https://doaj.org/article/d92f7a805d0a4aa697e8c1c2b9ffabca2020-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18726-9https://doaj.org/toc/2041-1723Determining molecular clustering in Photoactivated Localization Microscopy (PALM) experiments requires knowledge of the blinking properties of the fluorophore to prevent overcounting artefacts. Here the authors develop an experimental and analytical framework to determine the blinking parameters of fluorophores and incorporate this information into cluster analysis.René PlatzerBenedikt K. RossbothMagdalena C. SchneiderEva SevcsikFlorian BaumgartHannes StockingerGerhard J. SchützJohannes B. HuppaMario BrameshuberNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q René Platzer Benedikt K. Rossboth Magdalena C. Schneider Eva Sevcsik Florian Baumgart Hannes Stockinger Gerhard J. Schütz Johannes B. Huppa Mario Brameshuber Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
description	Determining molecular clustering in Photoactivated Localization Microscopy (PALM) experiments requires knowledge of the blinking properties of the fluorophore to prevent overcounting artefacts. Here the authors develop an experimental and analytical framework to determine the blinking parameters of fluorophores and incorporate this information into cluster analysis.
format	article
author	René Platzer Benedikt K. Rossboth Magdalena C. Schneider Eva Sevcsik Florian Baumgart Hannes Stockinger Gerhard J. Schütz Johannes B. Huppa Mario Brameshuber
author_facet	René Platzer Benedikt K. Rossboth Magdalena C. Schneider Eva Sevcsik Florian Baumgart Hannes Stockinger Gerhard J. Schütz Johannes B. Huppa Mario Brameshuber
author_sort	René Platzer
title	Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
title_short	Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
title_full	Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
title_fullStr	Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
title_full_unstemmed	Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
title_sort	unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d92f7a805d0a4aa697e8c1c2b9ffabca
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Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy

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