Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
Determining molecular clustering in Photoactivated Localization Microscopy (PALM) experiments requires knowledge of the blinking properties of the fluorophore to prevent overcounting artefacts. Here the authors develop an experimental and analytical framework to determine the blinking parameters of...
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Auteurs principaux: | René Platzer, Benedikt K. Rossboth, Magdalena C. Schneider, Eva Sevcsik, Florian Baumgart, Hannes Stockinger, Gerhard J. Schütz, Johannes B. Huppa, Mario Brameshuber |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2020
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Sujets: | |
Accès en ligne: | https://doaj.org/article/d92f7a805d0a4aa697e8c1c2b9ffabca |
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