Protocol for design, construction, and selection of genome phage (gPhage) display libraries

Protocol for design, construction, and selection of genome phage (gPhage) display libraries

Summary: This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape a...

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Bibliographic Details
Main Authors: Luis Antonio Rodriguez Carnero, André Azevedo Reis Teixeira, Fenny Hui Fen Tang, Andréia Kuramoto, Maria Júlia Manso Alves, Walter Colli, João Carlos Setubal, Edécio Cunha-Neto, Renata Pasqualini, Wadih Arap, Ricardo José Giordano
Format: article
Language:EN
Published: Elsevier 2021
Subjects:
Sequence analysis
Genomics
Sequencing
High Throughput Screening
Immunology
Microbiology
Science (General)
Q1-390
Online Access:https://doaj.org/article/d9424f25152a4e7cb7d4a8716980ff32
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https://doaj.org/article/d9424f25152a4e7cb7d4a8716980ff32

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